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Extracellular matrix of galectin-1-exposed dermal and tumor-associated fibroblasts favors growth of human umbilical vein endothelial cells in vitro: a short report

V. Peržeľová, L. Varinská, B. Dvořánková, P. Szabo, P. Spurný, J. Valach, J. Mojžiš, S. André, HJ. Gabius, K. Smetana, P. Gál,

. 2014 ; 34 (8) : 3991-6.

Jazyk angličtina Země Řecko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15007971

BACKGROUND/AIM: Stromal cells in the tumor microenvironment are primarily considered as sources of promalignant factors. The objective of our study was to define the effect of extracellular matrix (ECM) produced by normal dermal or cancer-associated fibroblasts exposed to adhesion/growth-regulatory lectin galectin-1 on human umbilical vein endothelial cells (HUVECs). MATERIALS AND METHODS: Fibroblasts were cultured for 10 days with lectin, followed by removing cellular constituents after an osmotic shock. Freshly-isolated HUVECs were placed on the ECM. In parallel, HUVECs were seeded on untreated and gelatin-coated surfaces as controls. A positive control for growth of HUVECs culture using medium supplemented with vascular endothelial growth factor completed the test panel. Cells were kept in contact to the substratum for two days and then processed for immunocytochemistry. RESULTS: HUVECs seeded on fibroblast-generated ECM presented a comparatively high degree of proliferation. Furthermore, contact to substratum produced by tumor-associated fibroblasts led to generation of a meshwork especially rich in fibronectin. CONCLUSION: Galectin-1 is apparently capable to trigger ECM production favorable for growth of HUVECs, prompting further work on characterizing structural features of the ECM and in situ correlation of lectin presence, ECM constitution and neoangiogenesis.

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$a Peržeľová, Vlasta $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic Department of Pathological Anatomy, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
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$a Extracellular matrix of galectin-1-exposed dermal and tumor-associated fibroblasts favors growth of human umbilical vein endothelial cells in vitro: a short report / $c V. Peržeľová, L. Varinská, B. Dvořánková, P. Szabo, P. Spurný, J. Valach, J. Mojžiš, S. André, HJ. Gabius, K. Smetana, P. Gál,
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$a BACKGROUND/AIM: Stromal cells in the tumor microenvironment are primarily considered as sources of promalignant factors. The objective of our study was to define the effect of extracellular matrix (ECM) produced by normal dermal or cancer-associated fibroblasts exposed to adhesion/growth-regulatory lectin galectin-1 on human umbilical vein endothelial cells (HUVECs). MATERIALS AND METHODS: Fibroblasts were cultured for 10 days with lectin, followed by removing cellular constituents after an osmotic shock. Freshly-isolated HUVECs were placed on the ECM. In parallel, HUVECs were seeded on untreated and gelatin-coated surfaces as controls. A positive control for growth of HUVECs culture using medium supplemented with vascular endothelial growth factor completed the test panel. Cells were kept in contact to the substratum for two days and then processed for immunocytochemistry. RESULTS: HUVECs seeded on fibroblast-generated ECM presented a comparatively high degree of proliferation. Furthermore, contact to substratum produced by tumor-associated fibroblasts led to generation of a meshwork especially rich in fibronectin. CONCLUSION: Galectin-1 is apparently capable to trigger ECM production favorable for growth of HUVECs, prompting further work on characterizing structural features of the ECM and in situ correlation of lectin presence, ECM constitution and neoangiogenesis.
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$a Varinská, Lenka $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic.
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$a Dvořánková, Barbora $u Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
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$a Szabo, Pavol $u Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
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$a Spurný, Peter $u Department of Cardiology, East-Slovak Institute of Cardiovascular Diseases Inc., Košice, Slovak Republic.
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$a Valach, Jaroslav $u Department of Stomatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
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$a Mojžiš, Ján $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic.
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$a André, Sabine $u Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilian University, Munich, Germany. $7 gn_A_00006374
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$a Gabius, Hans-Joachim $u Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilian University, Munich, Germany.
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$a Smetana, Karel $u Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic galovci@yahoo.com pgal@vusch.sk karel.smetana@lf1.cuni.cz.
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$a Gál, Peter $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic Department of Biomedical Research, East-Slovak Institute of Cardiovascular Diseases Inc., Košice, Slovak Republic galovci@yahoo.com pgal@vusch.sk karel.smetana@lf1.cuni.cz.
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