-
Something wrong with this record ?
The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling
T. Balážová, J. Makovcová, O. Šedo, M. Slaný, M. Faldyna, Z. Zdráhal,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Culture Media pharmacology MeSH
- Mycobacterium drug effects MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Bacterial Typing Techniques MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacterium smegmatis. Optimum culture conditions yielding the highest identification success rate against the BioTyper database (Bruker Daltonics) and permitting the possibility of automatic acquisition of mass spectra were found to be distinct for the two mycobacterial species examined. Similarly, individual changes in growth conditions had diverse effects on the two species. For these reasons, thorough control over cultivation conditions should always be employed to maximize the performance and discriminatory power of MALDI-TOF MS profiling, and cultivation conditions must be optimized separately for individual groups of mycobacterial species/strains.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15008267
- 003
- CZ-PrNML
- 005
- 20150325100153.0
- 007
- ta
- 008
- 150306s2014 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1111/1574-6968.12408 $2 doi
- 035 __
- $a (PubMed)24571790
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Balážová, Tereza $u Research Group Proteomics, CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Department of Microbiology, Faculty of Science, Masaryk University, Brno, Czech Republic.
- 245 14
- $a The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling / $c T. Balážová, J. Makovcová, O. Šedo, M. Slaný, M. Faldyna, Z. Zdráhal,
- 520 9_
- $a Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacterium smegmatis. Optimum culture conditions yielding the highest identification success rate against the BioTyper database (Bruker Daltonics) and permitting the possibility of automatic acquisition of mass spectra were found to be distinct for the two mycobacterial species examined. Similarly, individual changes in growth conditions had diverse effects on the two species. For these reasons, thorough control over cultivation conditions should always be employed to maximize the performance and discriminatory power of MALDI-TOF MS profiling, and cultivation conditions must be optimized separately for individual groups of mycobacterial species/strains.
- 650 _2
- $a techniky typizace bakterií $7 D015373
- 650 _2
- $a kultivační média $x farmakologie $7 D003470
- 650 _2
- $a Mycobacterium $x účinky léků $7 D009161
- 650 _2
- $a spektrometrie hmotnostní - ionizace laserem za účasti matrice $x metody $7 D019032
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Makovcová, Jitka
- 700 1_
- $a Šedo, Ondrej
- 700 1_
- $a Slaný, Michal
- 700 1_
- $a Faldyna, Martin
- 700 1_
- $a Zdráhal, Zbyněk
- 773 0_
- $w MED00001792 $t FEMS microbiology letters $x 1574-6968 $g Roč. 353, č. 1 (2014), s. 77-84
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/24571790 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20150306 $b ABA008
- 991 __
- $a 20150325100442 $b ABA008
- 999 __
- $a ok $b bmc $g 1065540 $s 891067
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 353 $c 1 $d 77-84 $i 1574-6968 $m FEMS microbiology letters $n FEMS Microbiol Lett $x MED00001792
- LZP __
- $a Pubmed-20150306
