The limited specimen tilting range that is typically available in electron tomography gives rise to a region in the Fourier space of the reconstructed object where experimental data are unavailable - the missing wedge. Since this region is sharply delimited from the area of available data, the reconstructed signal is typically hampered by convolution with its impulse response, which gives rise to the well-known missing wedge artefacts in 3D reconstructions. Despite the recent progress in the field of reconstruction and regularization techniques, the missing wedge artefacts remain untreated in most current reconstruction workflows in structural biology. Therefore we have designed a simple Fourier angular filter that effectively suppresses the ray artefacts in the single-axis tilting projection acquisition scheme, making single-axis tomographic reconstructions easier to interpret in particular at low signal-to-noise ratio in acquired projections. The proposed filter can be easily incorporated into current electron tomographic reconstruction schemes.

Centre for Biomedical Image Analysis Faculty of Informatics Masaryk University Brno Czech Republic

Charles University Prague 1st Faculty of Medicine Institute of Cellular Biology and Pathology Albertov 4 128 01 Prague 2 Czech Republic

MPI CBG Photenhauerstr 108 01307 Dresden Germany

Sir William Dunn School of Pathology University of Oxford South Parks Road Oxford OX1 3RE UK

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