The main analytical benefit of this study is the development of methods enabling a rapid determination of total lipids of algae by lipidomic analysis and detailed identification and quantification of a complex mixture of natural TAGs by silver-LC/APCI-MS and NARP-LC/APCI-MS. Both types of chromatography can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:3 and 16:4 acids in the molecule. We conclude that the genus Chloromonas is a major producer of C16 PUFAs mostly contained in TAGs. Since more detailed studies in this field have been stymied by the shortage of 16:3 and 16:4 FAs, we decided to study the alga Chloromonas as a potential biotechnological source of C16 PUFAs.

Charles University Prague Faculty of Science Department of Ecology Viničná 7 128 44 Prague 2 Czech Republic

Institute of Microbiology Academy of Sciences of the Czech Republic Vídeňská 1083 142 20 Prague Czech Republic

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$a Rezanka, Tomáš $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic. Electronic address: rezanka@biomed.cas.cz.

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$a Lipidomic profiling of snow algae by ESI-MS and silver-LC/APCI-MS / $c T. Rezanka, L. Nedbalová, L. Procházková, K. Sigler,

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$a The main analytical benefit of this study is the development of methods enabling a rapid determination of total lipids of algae by lipidomic analysis and detailed identification and quantification of a complex mixture of natural TAGs by silver-LC/APCI-MS and NARP-LC/APCI-MS. Both types of chromatography can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:3 and 16:4 acids in the molecule. We conclude that the genus Chloromonas is a major producer of C16 PUFAs mostly contained in TAGs. Since more detailed studies in this field have been stymied by the shortage of 16:3 and 16:4 FAs, we decided to study the alga Chloromonas as a potential biotechnological source of C16 PUFAs.

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$a Nedbalová, Linda $u Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 128 44 Prague 2, Czech Republic.

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$a Procházková, Lenka $u Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 128 44 Prague 2, Czech Republic.

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$a Sigler, Karel $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic.

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