The main analytical benefit of this study is the development of methods enabling a rapid determination of total lipids of algae by lipidomic analysis and detailed identification and quantification of a complex mixture of natural TAGs by silver-LC/APCI-MS and NARP-LC/APCI-MS. Both types of chromatography can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:3 and 16:4 acids in the molecule. We conclude that the genus Chloromonas is a major producer of C16 PUFAs mostly contained in TAGs. Since more detailed studies in this field have been stymied by the shortage of 16:3 and 16:4 FAs, we decided to study the alga Chloromonas as a potential biotechnological source of C16 PUFAs.

Charles University Prague Faculty of Science Department of Ecology Viničná 7 128 44 Prague 2 Czech Republic

Institute of Microbiology Academy of Sciences of the Czech Republic Vídeňská 1083 142 20 Prague Czech Republic

Unicellular versus Filamentous: The Glacial Alga Ancylonema alaskana comb. et stat. nov. and Its Ecophysiological Relatedness to Ancylonema nordenskioeldii (Zygnematophyceae, Streptophyta)

Cysts of the Snow Alga Chloromonas krienitzii (Chlorophyceae) Show Increased Tolerance to Ultraviolet Radiation and Elevated Visible Light

Ecophysiological and morphological comparison of two populations of Chlainomonas sp. (Chlorophyta) causing red snow on ice-covered lakes in the High Tatras and Austrian Alps

Chloromonas nivalis subsp. tatrae, subsp. nov. (Chlamydomonadales, Chlorophyta): re-examination of a snow alga from the High Tatra Mountains (Slovakia)

Ecophysiology, secondary pigments and ultrastructure of Chlainomonas sp. (Chlorophyta) from the European Alps compared with Chlamydomonas nivalis forming red snow

Separation of enantiomeric triacylglycerols by chiral-phase HPLC