Lipidomic profiling of snow algae by ESI-MS and silver-LC/APCI-MS
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Odkazy
PubMed
24548555
DOI
10.1016/j.phytochem.2014.01.017
PII: S0031-9422(14)00041-7
Knihovny.cz E-zdroje
- Klíčová slova
- Chloromonas pichinchae, ESI, Lipidomics, NARP-LC/APCI-MS, Silver-LC/APCI-MS, Snow alga, Triacylglycerols,
- MeSH
- chromatografie kapalinová metody MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- lipidy analýza chemie MeSH
- stříbro chemie MeSH
- triglyceridy analýza chemie MeSH
- Volvocida chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- lipidy MeSH
- stříbro MeSH
- triglyceridy MeSH
The main analytical benefit of this study is the development of methods enabling a rapid determination of total lipids of algae by lipidomic analysis and detailed identification and quantification of a complex mixture of natural TAGs by silver-LC/APCI-MS and NARP-LC/APCI-MS. Both types of chromatography can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:3 and 16:4 acids in the molecule. We conclude that the genus Chloromonas is a major producer of C16 PUFAs mostly contained in TAGs. Since more detailed studies in this field have been stymied by the shortage of 16:3 and 16:4 FAs, we decided to study the alga Chloromonas as a potential biotechnological source of C16 PUFAs.
Separation of enantiomeric triacylglycerols by chiral-phase HPLC