Lipidomic profiling of snow algae by ESI-MS and silver-LC/APCI-MS
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24548555
DOI
10.1016/j.phytochem.2014.01.017
PII: S0031-9422(14)00041-7
Knihovny.cz E-resources
- Keywords
- Chloromonas pichinchae, ESI, Lipidomics, NARP-LC/APCI-MS, Silver-LC/APCI-MS, Snow alga, Triacylglycerols,
- MeSH
- Chromatography, Liquid methods MeSH
- Spectrometry, Mass, Electrospray Ionization methods MeSH
- Lipids analysis chemistry MeSH
- Silver chemistry MeSH
- Triglycerides analysis chemistry MeSH
- Volvocida chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Lipids MeSH
- Silver MeSH
- Triglycerides MeSH
The main analytical benefit of this study is the development of methods enabling a rapid determination of total lipids of algae by lipidomic analysis and detailed identification and quantification of a complex mixture of natural TAGs by silver-LC/APCI-MS and NARP-LC/APCI-MS. Both types of chromatography can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:3 and 16:4 acids in the molecule. We conclude that the genus Chloromonas is a major producer of C16 PUFAs mostly contained in TAGs. Since more detailed studies in this field have been stymied by the shortage of 16:3 and 16:4 FAs, we decided to study the alga Chloromonas as a potential biotechnological source of C16 PUFAs.
References provided by Crossref.org
Separation of enantiomeric triacylglycerols by chiral-phase HPLC
