The microRNA (miRNA) belongs to short noncoding RNA molecules (generally 20-25 nt in length) which are able of specific gene regulation expression 1. These regulatory molecules have significant influence on different biological processes like cell proliferation, development, differentiation, apoptosis and metabolism 2. The aberrant expression of various miRNAs is involved in the cell pathological status formation. The abnormal miRNA expression levels were found at number diseases like inflammatory and autoimmune diseases, diabetes, cardiovascular and neurodegenerative diseases and cancer 3-6. Specific miRNAs expression profiles were identified at various tumors so miRNA are interesting diagnostic biomarkers. Moreover their detection might be helpful to find more about cancer staging, prognosis and/or response to treatment 7. Different studies have shown that miR-150 is upregulated in lung cancer tissue and lupus nephritis, downregulated at patients with heart failure, acute myeloblastic leukemia, colorectal cancer and systemic sclerosis 8-14. Because of miRNAs importance the simple and fast detection method is needed. Therefore, the sensitive electrochemical analysis was combined with specific magnetic particles-based isolation.

Central European Institute of Technology Brno University of Technology

Department of Chemistry and Biochemistry Mendel University in Brno

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