Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Interaction of human osteoblast-like Saos-2 and MG-63 cells with thermally oxidized surfaces of a titanium-niobium alloy

M. Vandrovcova, I. Jirka, K. Novotna, V. Lisa, O. Frank, Z. Kolska, V. Stary, L. Bacakova,

. 2014 ; 9 (6) : e100475.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15014181

An investigation was made of the adhesion, growth and differentiation of osteoblast-like MG-63 and Saos-2 cells on titanium (Ti) and niobium (Nb) supports and on TiNb alloy with surfaces oxidized at 165°C under hydrothermal conditions and at 600°C in a stream of air. The oxidation mode and the chemical composition of the samples tuned the morphology, topography and distribution of the charge on their surfaces, which enabled us to evaluate the importance of these material characteristics in the interaction of the cells with the sample surface. Numbers of adhered MG-63 and Saos-2 cells correlated with the number of positively-charged (related with the Nb2O5 phase) and negatively-charged sites (related with the TiO2 phase) on the alloy surface. Proliferation of these cells is correlated with the presence of positively-charged (i.e. basic) sites of the Nb2O5 alloy phase, while cell differentiation is correlated with negatively-charged (acidic) sites of the TiO2 alloy phase. The number of charged sites and adhered cells was substantially higher on the alloy sample oxidized at 600°C than on the hydrothermally treated sample at 165°C. The expression values of osteoblast differentiation markers (collagen type I and osteocalcin) were higher for cells grown on the Ti samples than for those grown on the TiNb samples. This was more particularly apparent in the samples treated at 165°C. No considerable immune activation of murine macrophage-like RAW 264.7 cells on the tested samples was found. The secretion of TNF-α by these cells into the cell culture media was much lower than for either cells grown in the presence of bacterial lipopolysaccharide, or untreated control samples. Thus, oxidized Ti and TiNb are both promising materials for bone implantation; TiNb for applications where bone cell proliferation is desirable, and Ti for induction of osteogenic cell differentiation.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15014181
003      
CZ-PrNML
005      
20150421092129.0
007      
ta
008      
150420s2014 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0100475 $2 doi
035    __
$a (PubMed)24977704
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Vandrovcova, Marta $u Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
245    10
$a Interaction of human osteoblast-like Saos-2 and MG-63 cells with thermally oxidized surfaces of a titanium-niobium alloy / $c M. Vandrovcova, I. Jirka, K. Novotna, V. Lisa, O. Frank, Z. Kolska, V. Stary, L. Bacakova,
520    9_
$a An investigation was made of the adhesion, growth and differentiation of osteoblast-like MG-63 and Saos-2 cells on titanium (Ti) and niobium (Nb) supports and on TiNb alloy with surfaces oxidized at 165°C under hydrothermal conditions and at 600°C in a stream of air. The oxidation mode and the chemical composition of the samples tuned the morphology, topography and distribution of the charge on their surfaces, which enabled us to evaluate the importance of these material characteristics in the interaction of the cells with the sample surface. Numbers of adhered MG-63 and Saos-2 cells correlated with the number of positively-charged (related with the Nb2O5 phase) and negatively-charged sites (related with the TiO2 phase) on the alloy surface. Proliferation of these cells is correlated with the presence of positively-charged (i.e. basic) sites of the Nb2O5 alloy phase, while cell differentiation is correlated with negatively-charged (acidic) sites of the TiO2 alloy phase. The number of charged sites and adhered cells was substantially higher on the alloy sample oxidized at 600°C than on the hydrothermally treated sample at 165°C. The expression values of osteoblast differentiation markers (collagen type I and osteocalcin) were higher for cells grown on the Ti samples than for those grown on the TiNb samples. This was more particularly apparent in the samples treated at 165°C. No considerable immune activation of murine macrophage-like RAW 264.7 cells on the tested samples was found. The secretion of TNF-α by these cells into the cell culture media was much lower than for either cells grown in the presence of bacterial lipopolysaccharide, or untreated control samples. Thus, oxidized Ti and TiNb are both promising materials for bone implantation; TiNb for applications where bone cell proliferation is desirable, and Ti for induction of osteogenic cell differentiation.
650    _2
$a slitiny $x chemie $x farmakologie $7 D000497
650    _2
$a zvířata $7 D000818
650    _2
$a biologické markery $x metabolismus $7 D015415
650    _2
$a buněčná adheze $x účinky léků $7 D002448
650    _2
$a buněčná diferenciace $x účinky léků $7 D002454
650    _2
$a buněčné linie $7 D002460
650    _2
$a proliferace buněk $x účinky léků $7 D049109
650    _2
$a kolagen typu I $x metabolismus $7 D024042
650    _2
$a vysoká teplota $7 D006358
650    _2
$a lidé $7 D006801
650    _2
$a lipopolysacharidy $x farmakologie $7 D008070
650    _2
$a makrofágy $x cytologie $x účinky léků $x metabolismus $7 D008264
650    _2
$a myši $7 D051379
650    _2
$a osteoblasty $x cytologie $x účinky léků $x metabolismus $7 D010006
650    _2
$a osteokalcin $x metabolismus $7 D015675
650    _2
$a oxidace-redukce $7 D010084
650    _2
$a statická elektřina $7 D055672
650    _2
$a povrchové vlastnosti $7 D013499
650    12
$a tkáňové podpůrné struktury $7 D054457
650    _2
$a TNF-alfa $x farmakologie $7 D014409
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Jirka, Ivan $u J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Novotna, Katarina $u Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Lisa, Vera $u Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Frank, Otakar $u J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Kolska, Zdenka $u Faculty of Science, J.E. Purkinje University, Usti nad Labem, Czech Republic.
700    1_
$a Stary, Vladimir $u Faculty of Mechanical Engineering, Czech Technical University in Prague, Prague, Czech Republic.
700    1_
$a Bacakova, Lucie $u Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 9, č. 6 (2014), s. e100475
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24977704 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20150420 $b ABA008
991    __
$a 20150421092427 $b ABA008
999    __
$a ok $b bmc $g 1071762 $s 897059
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 9 $c 6 $d e100475 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$a Pubmed-20150420

Najít záznam

Citační ukazatele

Možnosti archivace