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Depolarization affects the lateral microdomain structure of yeast plasma membrane
P. Herman, J. Vecer, M. Opekarova, P. Vesela, I. Jancikova, J. Zahumensky, J. Malinsky,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu úvodníky, práce podpořená grantem
NLK
Free Medical Journals
od 2005 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2005-01-01 do Před 1 rokem
Wiley Free Content
od 2005 do Před 1 rokem
PubMed
25410771
DOI
10.1111/febs.13156
Knihovny.cz E-zdroje
- MeSH
- buněčná membrána metabolismus MeSH
- membránové mikrodomény metabolismus MeSH
- membránové potenciály fyziologie MeSH
- Saccharomyces cerevisiae - proteiny metabolismus MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- Publikační typ
- práce podpořená grantem MeSH
- úvodníky MeSH
We report the transmembrane voltage-induced lateral reorganization of highly-ordered lipid microdomains in the plasma membrane of living Saccharomyces cerevisiae. Using trans-parinaric acid (all-trans-9,11,13,15-octadecatetraenoic acid) as a probe of lipid order and different methods of membrane depolarization, we found that depolarization always invokes a significant reduction in the amount of gel-like microdomains in the membrane. Different depolarization mechanisms, including the application of ionophores, cell depolarization by an external electric field, depolarization by proton/hexose co-transport facilitated by HUP1 protein and a reduction of membrane potential caused by compromised respiration efficiency, yielded the same results independently of the yeast strain used. The data suggest that the voltage-induced reorganization of lateral membrane structure could play significant role in fast cellular response to acute stress conditions, as well as in other membrane microdomain-related regulatory mechanisms.
Citace poskytuje Crossref.org
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