Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells

RJ. Stanton, V. Prod'homme, MA. Purbhoo, M. Moore, RJ. Aicheler, M. Heinzmann, SM. Bailer, J. Haas, R. Antrobus, MP. Weekes, PJ. Lehner, B. Vojtesek, KL. Miners, S. Man, GS. Wilkie, AJ. Davison, EC. Wang, P. Tomasec, GW. Wilkinson,

. 2014 ; 16 (2) : 201-14.

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc15023135

Immune evasion genes help human cytomegalovirus (HCMV) establish lifelong persistence. Without immune pressure, laboratory-adapted HCMV strains have undergone genetic alterations. Among these, the deletion of the UL/b' domain is associated with loss of virulence. In a screen of UL/b', we identified pUL135 as a protein responsible for the characteristic cytopathic effect of clinical HCMV strains that also protected from natural killer (NK) and T cell attack. pUL135 interacted directly with abl interactor 1 (ABI1) and ABI2 to recruit the WAVE2 regulatory complex to the plasma membrane, remodel the actin cytoskeleton and dramatically reduce the efficiency of immune synapse (IS) formation. An intimate association between F-actin filaments in target cells and the IS was dispelled by pUL135 expression. Thus, F-actin in target cells plays a critical role in synaptogenesis, and this can be exploited by pathogens to protect against cytotoxic immune effector cells. An independent interaction between pUL135 and talin disrupted cell contacts with the extracellular matrix.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15023135
003      
CZ-PrNML
005      
20150730101053.0
007      
ta
008      
150709s2014 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.chom.2014.07.005 $2 doi
035    __
$a (PubMed)25121749
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Stanton, Richard J $u Institute of Infection & Immunity, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK. Electronic address: stantonrj@cf.ac.uk.
245    10
$a HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells / $c RJ. Stanton, V. Prod'homme, MA. Purbhoo, M. Moore, RJ. Aicheler, M. Heinzmann, SM. Bailer, J. Haas, R. Antrobus, MP. Weekes, PJ. Lehner, B. Vojtesek, KL. Miners, S. Man, GS. Wilkie, AJ. Davison, EC. Wang, P. Tomasec, GW. Wilkinson,
520    9_
$a Immune evasion genes help human cytomegalovirus (HCMV) establish lifelong persistence. Without immune pressure, laboratory-adapted HCMV strains have undergone genetic alterations. Among these, the deletion of the UL/b' domain is associated with loss of virulence. In a screen of UL/b', we identified pUL135 as a protein responsible for the characteristic cytopathic effect of clinical HCMV strains that also protected from natural killer (NK) and T cell attack. pUL135 interacted directly with abl interactor 1 (ABI1) and ABI2 to recruit the WAVE2 regulatory complex to the plasma membrane, remodel the actin cytoskeleton and dramatically reduce the efficiency of immune synapse (IS) formation. An intimate association between F-actin filaments in target cells and the IS was dispelled by pUL135 expression. Thus, F-actin in target cells plays a critical role in synaptogenesis, and this can be exploited by pathogens to protect against cytotoxic immune effector cells. An independent interaction between pUL135 and talin disrupted cell contacts with the extracellular matrix.
650    _2
$a mikrofilamenta $x metabolismus $7 D008841
650    _2
$a adaptorové proteiny signální transdukční $x metabolismus $7 D048868
650    _2
$a CD8-pozitivní T-lymfocyty $x imunologie $x virologie $7 D018414
650    _2
$a Cytomegalovirus $x imunologie $7 D003587
650    _2
$a cytoskeletální proteiny $x metabolismus $7 D003598
650    _2
$a interakce hostitele a patogenu $7 D054884
650    _2
$a lidé $7 D006801
650    _2
$a imunologické synapse $x virologie $7 D054992
650    _2
$a imunomodulace $7 D056747
650    _2
$a buňky NK $x imunologie $x virologie $7 D007694
650    _2
$a talin $x metabolismus $7 D016608
650    _2
$a virové proteiny $x fyziologie $7 D014764
650    _2
$a rodina proteinů Wiskottova-Aldrichova syndromu $x metabolismus $7 D051300
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Prod'homme, Virginie $u Institute of Infection & Immunity, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK.
700    1_
$a Purbhoo, Marco A $u Section of Hepatology, Department of Medicine, Imperial College London, London, W2 1PG, UK.
700    1_
$a Moore, Melanie $u Institute of Infection & Immunity, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK.
700    1_
$a Aicheler, Rebecca J $u Institute of Infection & Immunity, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK. $7 gn_A_00002657
700    1_
$a Heinzmann, Marcus $u Ludwig-Maximilians-Universität München, Max von Pettenkofer-Institut, Pettenkoferstrasse 9a, 80336 München, Germany.
700    1_
$a Bailer, Susanne M $u Ludwig-Maximilians-Universität München, Max von Pettenkofer-Institut, Pettenkoferstrasse 9a, 80336 München, Germany; Biological Interfacial Engineering, University of Stuttgart, Nobelstrasse 12, 70569 Stuttgart, Germany.
700    1_
$a Haas, Jürgen $u Ludwig-Maximilians-Universität München, Max von Pettenkofer-Institut, Pettenkoferstrasse 9a, 80336 München, Germany.
700    1_
$a Antrobus, Robin $u University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0XY, UK. $7 gn_A_00007582
700    1_
$a Weekes, Michael P $u University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0XY, UK.
700    1_
$a Lehner, Paul J $u University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0XY, UK.
700    1_
$a Vojtesek, Borivoj $u Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, 65653 Brno, Czech Republic.
700    1_
$a Miners, Kelly L $u Institute of Infection & Immunity, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK.
700    1_
$a Man, Stephen $u Institute of Cancer and Genetics, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK.
700    1_
$a Wilkie, Gavin S $u Medical Research Council, University of Glasgow Centre for Virus Research, Glasgow G11 5JR, UK.
700    1_
$a Davison, Andrew J $u Medical Research Council, University of Glasgow Centre for Virus Research, Glasgow G11 5JR, UK.
700    1_
$a Wang, Eddie C Y $u Institute of Infection & Immunity, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK.
700    1_
$a Tomasec, Peter $u Institute of Infection & Immunity, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK.
700    1_
$a Wilkinson, Gavin W G $u Institute of Infection & Immunity, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK. Electronic address: wilkinsongw1@cf.ac.uk.
773    0_
$w MED00173236 $t Cell host & microbe $x 1934-6069 $g Roč. 16, č. 2 (2014), s. 201-14
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25121749 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20150709 $b ABA008
991    __
$a 20150730101141 $b ABA008
999    __
$a ok $b bmc $g 1083473 $s 906128
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 16 $c 2 $d 201-14 $i 1934-6069 $m Cell host & microbe $n Cell Host Microbe $x MED00173236
LZP    __
$a Pubmed-20150709

Find record

Citation metrics

Archiving options