Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied. Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved. EPS synthesis and cell growth had different optima. For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %). As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased. Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS. Aeration intensity and time of cultivation had little effect on EPS composition. Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352. The row EPS was composed of acidic glucuronoxylomannan for more than 85 %. In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats. The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.

Contipro Group s r o Dolní Dobrouč 401 561 02 Dolní Dobrouč Czech Republic

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$a Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied. Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved. EPS synthesis and cell growth had different optima. For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %). As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased. Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS. Aeration intensity and time of cultivation had little effect on EPS composition. Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352. The row EPS was composed of acidic glucuronoxylomannan for more than 85 %. In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats. The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.

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