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A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real-time quantitative PCR
H. White, L. Deprez, P. Corbisier, V. Hall, F. Lin, S. Mazoua, S. Trapmann, A. Aggerholm, H. Andrikovics, S. Akiki, G. Barbany, N. Boeckx, A. Bench, M. Catherwood, JM. Cayuela, S. Chudleigh, T. Clench, D. Colomer, F. Daraio, S. Dulucq, J....
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 2000-01-01 do Před 1 rokem
Open Access Digital Library
od 1997-01-01
Medline Complete (EBSCOhost)
od 1997-01-01 do 2015-11-30
Nursing & Allied Health Database (ProQuest)
od 2000-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2000-01-01 do Před 1 rokem
Public Health Database (ProQuest)
od 2000-01-01 do Před 1 rokem
PubMed
25036192
DOI
10.1038/leu.2014.217
Knihovny.cz E-zdroje
- MeSH
- bcr-abl fúzní proteiny genetika metabolismus MeSH
- DNA MeSH
- genová dávka MeSH
- kalibrace MeSH
- klonování DNA MeSH
- kvantitativní polymerázová řetězová reakce normy MeSH
- lidé MeSH
- membránové transportní proteiny genetika MeSH
- messenger RNA metabolismus MeSH
- plazmidy genetika MeSH
- proteiny z Escherichia coli genetika MeSH
- protoonkogenní proteiny c-bcr genetika MeSH
- referenční standardy MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Serial quantification of BCR-ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR-ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08±0.13 × 10(6), 1.08±0.11 × 10(5), 1.03±0.10 × 10(4), 1.02±0.09 × 10(3), 1.04±0.10 × 10(2) and 10.0±1.5 copies/μl. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR-ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise a number of measured transcripts of e14a2 BCR-ABL1 and three control genes (ABL1, BCR and GUSB). The set of six plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (https://ec.europa.eu/jrc/en/reference-materials/catalogue/; CRM code ERM-AD623a-f).
] Department of Laboratory Medicine UZ Leuven Belgium [2] Department of Oncology KU Leuven Belgium
3 Medizinische Klinik Medizinische Fakultät Mannheim der Universität Heidelberg Mannheim Germany
Abteilung für Hämatologie und internistische Onkologie Universität Leipzig Leipzig Germany
Bristol Genetics Laboratory Southmead Hospital Bristol UK
Cancer Molecular Diagnostics St James's Hospital Dublin Ireland
Cancer Research Institute The Catholic University of Korea Seoul South Korea
Children's Cancer Research Institute LabDia Labordiagnostik and Medical University Vienna Austria
Combined Laboratories Derriford Hospital Plymouth UK
Department of Clinical and Biological Science University of Turin Turin Italy
Department of Clinical Genetics University and Regional Laboratories Lund Sweden
Department of Clinical Medicine and Surgery University 'Federico II' of Naples Naples Italy
Department of Experimental Diagnostic and Specialty Medicine University of Bologna Bologna Italy
Department of Genetics and Molecular Pathology SA Pathology Adelaide SA Australia
Department of Haematology Aarhus University Hospital Aarhus Denmark
Department of Haematology Royal Liverpool University Hospital Liverpool UK
Department of Hematology Oncology Jena University Hospital Jena Germany
Department of Immunology Erasmus MC Rotterdam The Netherlands
Department of Immunology Genetics and Pathology Uppsala University Uppsala Sweden
Department of Internal Medicine Hematology Oncology Goethe University Frankfurt Germany
Department of Laboratory Medicine Medical University of Vienna Vienna Austria
Department of Medical Genetics NHS Grampian Aberdeen UK
Department of Molecular Haematology Yorkhill NHS Trust Glasgow UK
Division of Pathology Rikshospital Oslo University Hospital Oslo Norway
Genetics Department Institute of Experimental Medicine Istanbul University Istanbul Turkey
Haematology Department Belfast City Hospital Belfast UK
Haematology Research Laboratory Biomedical Research Foundation Academy of Athens Athens Greece
Hematology Department and HCT Unit G Papanicolaou Hospital Thessaloniki Greece
Hematology Department Jagiellonian University Krakow Poland
Hematopathology Unit Hospital Clinic IDIBAPS Barcelona Spain
HMDS Leeds Institute of Oncology St James's University Hospital Leeds UK
Hungarian National Blood Transfusion Service Budapest Hungary
Imperial Molecular Pathology Centre for Haematology Imperial College London London UK
Institute of Hematology and Blood Transfusion Prague Czech Republic
Lab Hematologia Hospital de la Santa Creu i Sant Pau Barcelona Spain
Laboratoire d'hématologie CHU Lille Lille France
Laboratory for Molecular Haemato Oncology Kings College Hospital London UK
Laboratory of Hematology Sheba Medical Center Tel Hashomer Israel
Medical Genetics Department Erasme Hospital Brussels Belgium
Molecular Diagnostics Laboratory Department of Hematology University Hospital Bern Bern Switzerland
Molecular Diagnostics The Royal Marsden NHS Foundation Trust Sutton UK
Molecular Haematology Bristol Royal Infirmary Bristol UK
Molecular Oncology Diagnostics Unit Guy's Hospital London UK
Molecular Pathology University Hospitals Southampton NHS Foundation Trust Southampton UK
National Genetics Reference Laboratory Salisbury District Hospital Salisbury UK
Regional Genetics Laboratory Birmingham Women's NHS Foundation Trust Birmingham UK
Sheffield Diagnostic Genetics Service Sheffield Children's NHS Foundation Trust Sheffield UK
Turku University Hospital TYKSLAB Laboratory of Molecular Genetics Turku Finland
United Laboratories of Tartu University Hospitals Tartu Estonia
VU Medical Centre Department of Haematology Amsterdam The Netherlands
Citace poskytuje Crossref.org
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