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Development and characterization of multiplex panels of polymorphic microsatellite loci in giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), using next-generation sequencing approach
G. Minárik, E. Bazsalovicsová, L. Zvijáková, J. Stefka, L. Pálková, I. Králová-Hromadová,
Language English Country Netherlands
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Fasciolidae classification genetics isolation & purification MeSH
- Trematode Infections parasitology veterinary MeSH
- Microsatellite Repeats MeSH
- Polymorphism, Genetic * MeSH
- Ruminants parasitology MeSH
- High-Throughput Nucleotide Sequencing methods MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
The microsatellite markers were designed for the giant liver fluke, Fascioloides magna, veterinary important liver parasite of free-living and domestic ruminants. Due to its geographic distribution (five enzootic regions across USA and Canada, three permanent European foci) and invasive character, F. magna is an interesting model for population genetics. Out of 667 amplicon candidates generated after NGS, 118 provided the best resolution and were tested with PCR analysis. In total, 56 yielded PCR products of expected size and in 36 of them the declared repetitive motif was identified by Sanger sequencing. After fragment analysis, 12 loci were proved to be polymorphic in individuals from one tested European and four North American populations. These loci were selected for setup of multiplex STR assays and utilized in genotyping of larger sample cohort. The outputs of statistical analyses indicate further global application of 11 conclusive loci in population genetics of the parasite.
Geneton Ltd Ilkovičova 3 84104 Bratislava Slovakia
Institute of Parasitology Slovak Academy of Sciences Hlinkova 3 04001 Košice Slovakia
References provided by Crossref.org
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- $a Minárik, Gabriel $u Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B-2, 84215 Bratislava, Slovakia; Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 81108 Bratislava, Slovakia; Geneton Ltd., Ilkovičova 3, 84104 Bratislava, Slovakia.
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