• Je něco špatně v tomto záznamu ?

Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity

SM. Alavi, P. Postlerová-Maňásková, A. Hatef, M. Pšenička, J. Pěknicová, K. Inaba, A. Ciereszko, O. Linhart,

. 2014 ; 40 (5) : 1393-8.

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15023515
E-zdroje Online Plný text

NLK ProQuest Central od 1997-01-01 do Před 1 rokem
Medline Complete (EBSCOhost) od 2003-03-01 do Před 1 rokem
Health & Medicine (ProQuest) od 1997-01-01 do Před 1 rokem

Odkazy

PubMed 24677048
DOI 10.1007/s10695-014-9933-8
Knihovny.cz E-zdroje

In mammals, proteases are present in sperm acrosome and play key role in fertilization. Sturgeon sperm has an acrosome, but its physiology, biochemistry, and potential role in fertilization are unknown. In the present study, we have observed high protease activity in acidic extract of intact sperm compared to that of seminal plasma in sterlet (Acipenser ruthenus). The protease activity was decreased and increased in acidic extract of motility-activated sperm and in the activation medium, respectively. Molecular analysis revealed total protease and serine (acrosin) protease activities in sperm acidic extract which was accumulated in a protein band with relative molecular mass of 35 kDa. Immunoelectron microscopy using an affinity-purified polyclonal antibody for boar acrosin localized the protease at the acrosome region. Moreover, initiation of sperm motility was inhibited after activation in the presence of inhibitors for both trypsin-like and chymotrypsin-like proteases, while the effects of protease inhibitors on sperm velocity were uncertain. Our results indicate similarities in physiology and biochemistry of acrosome between sturgeon and mammals and suggest potential role of protease in the initiation of sperm motility in sturgeon.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15023515
003      
CZ-PrNML
005      
20150728110501.0
007      
ta
008      
150709s2014 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s10695-014-9933-8 $2 doi
035    __
$a (PubMed)24677048
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Alavi, Sayyed Mohammad Hadi $u Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, 389 25, Vodňany, Czech Republic, alavi@af.czu.cz. $7 gn_A_00003328
245    10
$a Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity / $c SM. Alavi, P. Postlerová-Maňásková, A. Hatef, M. Pšenička, J. Pěknicová, K. Inaba, A. Ciereszko, O. Linhart,
520    9_
$a In mammals, proteases are present in sperm acrosome and play key role in fertilization. Sturgeon sperm has an acrosome, but its physiology, biochemistry, and potential role in fertilization are unknown. In the present study, we have observed high protease activity in acidic extract of intact sperm compared to that of seminal plasma in sterlet (Acipenser ruthenus). The protease activity was decreased and increased in acidic extract of motility-activated sperm and in the activation medium, respectively. Molecular analysis revealed total protease and serine (acrosin) protease activities in sperm acidic extract which was accumulated in a protein band with relative molecular mass of 35 kDa. Immunoelectron microscopy using an affinity-purified polyclonal antibody for boar acrosin localized the protease at the acrosome region. Moreover, initiation of sperm motility was inhibited after activation in the presence of inhibitors for both trypsin-like and chymotrypsin-like proteases, while the effects of protease inhibitors on sperm velocity were uncertain. Our results indicate similarities in physiology and biochemistry of acrosome between sturgeon and mammals and suggest potential role of protease in the initiation of sperm motility in sturgeon.
650    _2
$a akrosin $x metabolismus $7 D000176
650    _2
$a akrozom $x enzymologie $7 D000177
650    _2
$a analýza rozptylu $7 D000704
650    _2
$a zvířata $7 D000818
650    _2
$a ryby $x fyziologie $7 D005399
650    _2
$a histologické techniky $x veterinární $7 D006652
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a imunoelektronová mikroskopie $x veterinární $7 D016253
650    _2
$a proteasy $x farmakologie $7 D010447
650    _2
$a inhibitory proteas $x farmakologie $7 D011480
650    _2
$a rosanilinová barviva $7 D012394
650    _2
$a sperma $x enzymologie $7 D012661
650    _2
$a motilita spermií $x účinky léků $x fyziologie $7 D013081
650    _2
$a spermie $x účinky léků $x enzymologie $x fyziologie $7 D013094
650    _2
$a neparametrická statistika $7 D018709
650    _2
$a tosyllysinchlormethylketon $x farmakologie $7 D014107
650    _2
$a tosylfenylalanylchlormethylketon $x farmakologie $7 D014108
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Postlerová-Maňásková, Pavla
700    1_
$a Hatef, Azadeh
700    1_
$a Pšenička, Martin
700    1_
$a Pěknicová, Jana
700    1_
$a Inaba, Kazuo
700    1_
$a Ciereszko, Andrzej
700    1_
$a Linhart, Otomar
773    0_
$w MED00007610 $t Fish physiology and biochemistry $x 1573-5168 $g Roč. 40, č. 5 (2014), s. 1393-8
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24677048 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20150709 $b ABA008
991    __
$a 20150728110546 $b ABA008
999    __
$a ok $b bmc $g 1083852 $s 906508
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 40 $c 5 $d 1393-8 $i 1573-5168 $m Fish physiology and biochemistry $n Fish Physiol Biochem $x MED00007610
LZP    __
$a Pubmed-20150709

Najít záznam

Citační ukazatele

    Možnosti archivace