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Fifteen novel immunoreactive proteins of Chinese virulent Haemophilus parasuis serotype 5 verified by an immunoproteomic assay
Y. Yu, G. Wu, Z. Zhai, H. Yao, C. Lu, W. Zhang,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- 2D gelová elektroforéza MeSH
- bakteriální proteiny chemie genetika imunologie MeSH
- Haemophilus parasuis chemie klasifikace genetika imunologie MeSH
- hemofilové infekce imunologie mikrobiologie veterinární MeSH
- hmotnostní spektrometrie MeSH
- králíci MeSH
- molekulární sekvence - údaje MeSH
- nemoci prasat imunologie mikrobiologie MeSH
- prasata MeSH
- proteomika MeSH
- protilátky bakteriální analýza imunologie MeSH
- séroskupina MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Čína MeSH
Haemophilus parasuis (H. parasuis) is associated with meningitis, polyserositis, polyarthritis and bacterial pneumonia. At present, its prevention and control is difficult because of the lack of suitable subunit vaccines. Nowadays, high-throughput methods, immunoproteomics, are available to screen for more vaccine candidates. A protein extraction method for H. parasuis and two-dimensional electrophoresis (2-DE) were optimized to provide high-resolution profiles covering pH 3 to 10. Twenty immunoreactive spots were excised from gels after strict comparison between 2-DE Western blot membranes and the relevant gels. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and MALDI-TOF-TOF-MS successfully identified 16 different proteins. Fifteen of them were reported as immunoreactive proteins in H. parasuis for the first time. In addition, recombinant HP5-7 (ABC transporter, periplasmic-binding protein) showed immunoreactivity both with hyperimmune rabbit serum and convalescent swine serum. Four recombinants of the 14 successfully expressed genes showed immunoreactivity with hyperimmune rabbit serum.
Key Lab of Animal Bacteriology Ministry of Agriculture Nanjing Agricultural University Nanjing China
Citace poskytuje Crossref.org
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