-
Something wrong with this record ?
Chicken and rabbit antibodies against porcine pepsinogen A
L. Kulhavá, E. Miarková, P. Přikryl, P. Hodek, M. Tichá, Z. Kučerová
Language English Country Czech Republic
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Directory of Open Access Journals
from 2012
Medline Complete (EBSCOhost)
from 2012-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2013
- MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Immunohistochemistry MeSH
- Rabbits MeSH
- Chickens MeSH
- Stomach Neoplasms immunology metabolism MeSH
- Pepsinogen A immunology metabolism MeSH
- Swine MeSH
- Antibodies immunology MeSH
- Cattle MeSH
- Gastric Mucosa immunology metabolism pathology MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Isolated porcine pepsinogen A was used for the preparation of polyclonal rabbit and polyclonal chicken anti-pepsinogen A antibodies. Immunochemical properties of both immunoglobulin fractions were compared. The rabbit anti-serum was further purified using immobilized porcine pepsinogen A on magnetic cellulose beads and the resulting anti-pepsinogen A fraction proved to be applicable for the separation and the determination of porcine pepsinogen A. In contrary, antibodies prepared from chicken eggs by the same way have been found not suitable for the evaluation of the pepsinogen A level. Unexpectedly, the pre-immune fraction of chicken antibodies showed reactivity against porcine pepsinogen A and the affinity separation of specific polyclonal chicken anti-pepsinogen A antibodies on immobilized porcine pepsinogen A did not result in an enrichment of anti-pepsinogen A antibodies.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15026881
- 003
- CZ-PrNML
- 005
- 20150820075540.0
- 007
- ta
- 008
- 150812s2015 xr d f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.14712/23362936.2015.41 $2 doi
- 035 __
- $a (PubMed)25923966
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Kulhavá, Lucie $u Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic $7 xx0239734
- 245 10
- $a Chicken and rabbit antibodies against porcine pepsinogen A / $c L. Kulhavá, E. Miarková, P. Přikryl, P. Hodek, M. Tichá, Z. Kučerová
- 520 9_
- $a Isolated porcine pepsinogen A was used for the preparation of polyclonal rabbit and polyclonal chicken anti-pepsinogen A antibodies. Immunochemical properties of both immunoglobulin fractions were compared. The rabbit anti-serum was further purified using immobilized porcine pepsinogen A on magnetic cellulose beads and the resulting anti-pepsinogen A fraction proved to be applicable for the separation and the determination of porcine pepsinogen A. In contrary, antibodies prepared from chicken eggs by the same way have been found not suitable for the evaluation of the pepsinogen A level. Unexpectedly, the pre-immune fraction of chicken antibodies showed reactivity against porcine pepsinogen A and the affinity separation of specific polyclonal chicken anti-pepsinogen A antibodies on immobilized porcine pepsinogen A did not result in an enrichment of anti-pepsinogen A antibodies.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a protilátky $x imunologie $7 D000906
- 650 _2
- $a skot $7 D002417
- 650 _2
- $a kur domácí $7 D002645
- 650 _2
- $a ELISA $7 D004797
- 650 _2
- $a žaludeční sliznice $x imunologie $x metabolismus $x patologie $7 D005753
- 650 _2
- $a imunohistochemie $7 D007150
- 650 _2
- $a pepsinogen A $x imunologie $x metabolismus $7 D020116
- 650 _2
- $a králíci $7 D011817
- 650 _2
- $a nádory žaludku $x imunologie $x metabolismus $7 D013274
- 650 _2
- $a prasata $7 D013552
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Miarková, Eva $u Institute of Pathological Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic $7 _AN055715
- 700 1_
- $a Přikryl, Petr $u Institute of Pathological Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic $7 _BN004905
- 700 1_
- $a Hodek, Petr, $u Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic $d 1959- $7 jo2003186329
- 700 1_
- $a Tichá, Marie, $u Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic $d 1938-2017 $7 jn20000710640
- 700 1_
- $a Kučerová, Zdeňka $u Institute of Pathological Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic $7 _AN060068
- 773 0_
- $w MED00013414 $t Prague medical report $x 1214-6994 $g Roč. 116, č. 1 (2015), s. 16-23
- 856 41
- $u https://pmr.lf1.cuni.cz/media/pdf/pmr_2015116010016.pdf $y plný text volně přístupný
- 910 __
- $a ABA008 $b A 7 $c 1071 $y 4 $z 0
- 990 __
- $a 20150812 $b ABA008
- 991 __
- $a 20150819102932 $b ABA008
- 999 __
- $a ok $b bmc $g 1088286 $s 909919
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 116 $c 1 $d 16-23 $i 1214-6994 $m Prague Medical Report $n Prague Med. Rep. $x MED00013414
- LZP __
- $b NLK118 $a Pubmed-20150812
