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Up-regulation of orexigenic and down-regulation of anorexigenic neuropeptide gene expression in rat hypothalamus after partial lipectomy
Agnieszka Dettlaff-Pokora, Tomasz Sledzinski, Julian Swierczynski
Jazyk angličtina Země Česko
Typ dokumentu práce podpořená grantem
- MeSH
- AGRP protein genetika izolace a purifikace metabolismus MeSH
- bílá tuková tkáň chirurgie metabolismus účinky léků MeSH
- biomedicínský výzkum metody trendy MeSH
- experimenty na zvířatech MeSH
- hypothalamus * účinky léků zranění MeSH
- kokain analogy a deriváty izolace a purifikace MeSH
- leptin izolace a purifikace krev MeSH
- lipektomie * metody využití MeSH
- neuropeptid Y genetika metabolismus MeSH
- peptidové hormony genetika izolace a purifikace metabolismus MeSH
- polymerázová řetězová reakce s reverzní transkripcí metody využití MeSH
- potkani Wistar MeSH
- pro-opiomelanokortin genetika izolace a purifikace metabolismus MeSH
- regulace genové exprese * fyziologie genetika účinky léků MeSH
- statistika jako téma MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
Leptin down-regulates orexigenic and up-regulates anorexigenic neuropeptide gene expression in hypothalamus. Surgical removal of adipose tissue leads to decrease in circulating leptin concentrations in rats. In the present study, we tested: (a) regulation of neuropeptide gene expression in hypothalamus, (b) food intake, and (c) standard growth rate after removal of adipose tissue in rats. Partial lipectomy caused an approximately 10-fold reduction of subcutaneous, retroperitoneal and epididymal adipose tissue weight (at the end of experiments adipose tissue weight was 1.5 ± 0.9 in lipectomy and 15 ± 3.9 g in control rats; statistically significant). Compared to control rats, the animals subjected to lipectomy presented increased food intake, standard growth rate, and decreased serum leptin concentrations (2.6 ± 0.8 vs. 3.7 ± 1.2 ng/mL in the controls, statistically significant). These changes were associated with approximately twofold increase in neuropeptide Y, threefold increase in agouti-related peptide (orexigenic neuropeptides) and about 50% decrease in pro-opiomelanocortin and cocaine–amphetamine-regulated transcript peptide (anorexigenic neuropeptides) mRNA levels in the hypothalamus. These results suggest that partial lipectomy, leading to a decrease in circulating leptin concentrations, may exert an effect on hypothalamic orexigenic and anorexigenic neuropeptide gene expression, and consequently modulate food intake and standard growth rate in rats.
Department of Biochemistry Medical University of Gdansk Gdansk Poland
Department of Pharmaceutical Biochemistry Medical University of Gdansk Gdansk Poland
Citace poskytuje Crossref.org
Literatura
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