Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Instability restricts signaling of multiple fibroblast growth factors

M. Buchtova, R. Chaloupkova, M. Zakrzewska, I. Vesela, P. Cela, J. Barathova, I. Gudernova, R. Zajickova, L. Trantirek, J. Martin, M. Kostas, J. Otlewski, J. Damborsky, A. Kozubik, A. Wiedlocha, P. Krejci,

. 2015 ; 72 (12) : 2445-59. [pub] 20150218

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15031405

Fibroblast growth factors (FGFs) deliver extracellular signals that govern many developmental and regenerative processes, but the mechanisms regulating FGF signaling remain incompletely understood. Here, we explored the relationship between intrinsic stability of FGF proteins and their biological activity for all 18 members of the FGF family. We report that FGF1, FGF3, FGF4, FGF6, FGF8, FGF9, FGF10, FGF16, FGF17, FGF18, FGF20, and FGF22 exist as unstable proteins, which are rapidly degraded in cell cultivation media. Biological activity of FGF1, FGF3, FGF4, FGF6, FGF8, FGF10, FGF16, FGF17, and FGF20 is limited by their instability, manifesting as failure to activate FGF receptor signal transduction over long periods of time, and influence specific cell behavior in vitro and in vivo. Stabilization via exogenous heparin binding, introduction of stabilizing mutations or lowering the cell cultivation temperature rescues signaling of unstable FGFs. Thus, the intrinsic ligand instability is an important elementary level of regulation in the FGF signaling system.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15031405
003      
CZ-PrNML
005      
20170620083132.0
007      
ta
008      
151005s2015 sz f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s00018-015-1856-8 $2 doi
035    __
$a (PubMed)25854632
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Buchtová, Marcela $u Department of Anatomy, Histology and Embryology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. $7 xx0043736
245    10
$a Instability restricts signaling of multiple fibroblast growth factors / $c M. Buchtova, R. Chaloupkova, M. Zakrzewska, I. Vesela, P. Cela, J. Barathova, I. Gudernova, R. Zajickova, L. Trantirek, J. Martin, M. Kostas, J. Otlewski, J. Damborsky, A. Kozubik, A. Wiedlocha, P. Krejci,
520    9_
$a Fibroblast growth factors (FGFs) deliver extracellular signals that govern many developmental and regenerative processes, but the mechanisms regulating FGF signaling remain incompletely understood. Here, we explored the relationship between intrinsic stability of FGF proteins and their biological activity for all 18 members of the FGF family. We report that FGF1, FGF3, FGF4, FGF6, FGF8, FGF9, FGF10, FGF16, FGF17, FGF18, FGF20, and FGF22 exist as unstable proteins, which are rapidly degraded in cell cultivation media. Biological activity of FGF1, FGF3, FGF4, FGF6, FGF8, FGF10, FGF16, FGF17, and FGF20 is limited by their instability, manifesting as failure to activate FGF receptor signal transduction over long periods of time, and influence specific cell behavior in vitro and in vivo. Stabilization via exogenous heparin binding, introduction of stabilizing mutations or lowering the cell cultivation temperature rescues signaling of unstable FGFs. Thus, the intrinsic ligand instability is an important elementary level of regulation in the FGF signaling system.
650    _2
$a zvířata $7 D000818
650    _2
$a nádory kostí $x genetika $x metabolismus $x patologie $7 D001859
650    _2
$a nádory prsu $x genetika $x metabolismus $x patologie $7 D001943
650    12
$a proliferace buněk $7 D049109
650    _2
$a chondrosarkom $x genetika $x metabolismus $x patologie $7 D002813
650    _2
$a cirkulární dichroismus $7 D002942
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a fibroblastové růstové faktory $x chemie $x klasifikace $x genetika $x metabolismus $7 D005346
650    _2
$a lidé $7 D006801
650    _2
$a mutantní proteiny $x chemie $x metabolismus $7 D050505
650    _2
$a mutace $x genetika $7 D009154
650    _2
$a stabilita proteinů $7 D055550
650    _2
$a krysa rodu Rattus $7 D051381
650    12
$a signální transdukce $7 D015398
650    _2
$a teplota $7 D013696
650    _2
$a nádorové buňky kultivované $7 D014407
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Chaloupkova, Radka
700    1_
$a Zakrzewska, Malgorzata
700    1_
$a Veselá, Ivana $7 xx0214541
700    1_
$a Cela, Petra
700    1_
$a Barathova, Jana
700    1_
$a Gudernova, Iva
700    1_
$a Zajickova, Renata
700    1_
$a Trantirek, Lukas
700    1_
$a Martin, Jorge
700    1_
$a Kostas, Michal
700    1_
$a Otlewski, Jacek
700    1_
$a Damborsky, Jiri
700    1_
$a Kozubik, Alois
700    1_
$a Wiedlocha, Antoni
700    1_
$a Krejci, Pavel
773    0_
$w MED00001078 $t Cellular and molecular life sciences CMLS $x 1420-9071 $g Roč. 72, č. 12 (2015), s. 2445-59
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25854632 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20151005 $b ABA008
991    __
$a 20170620083550 $b ABA008
999    __
$a ok $b bmc $g 1092281 $s 914531
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 72 $c 12 $d 2445-59 $e 20150218 $i 1420-9071 $m Cellular and molecular life sciences $n Cell Mol Life Sci $x MED00001078
LZP    __
$a Pubmed-20151005
Zpět

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace