Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria

PJ. Sample, L. Kořený, Z. Paris, KW. Gaston, MA. Rubio, IM. Fleming, S. Hinger, E. Horáková, PA. Limbach, J. Lukeš, JD. Alfonzo,

. 2015 ; 43 (8) : 4262-73. [pub] 20150406

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15031409

Establishment of the early genetic code likely required strategies to ensure translational accuracy and inevitably involved tRNA post-transcriptional modifications. One such modification, wybutosine/wyosine is crucial for translational fidelity in Archaea and Eukarya; yet it does not occur in Bacteria and has never been described in mitochondria. Here, we present genetic, molecular and mass spectromery data demonstrating the first example of wyosine in mitochondria, a situation thus far unique to kinetoplastids. We also show that these modifications are important for mitochondrial function, underscoring their biological significance. This work focuses on TyW1, the enzyme required for the most critical step of wyosine biosynthesis. Based on molecular phylogeny, we suggest that the kinetoplastids pathways evolved via gene duplication and acquisition of an FMN-binding domain now prevalent in TyW1 of most eukaryotes. These findings are discussed in the context of the extensive U-insertion RNA editing in trypanosome mitochondria, which may have provided selective pressure for maintenance of mitochondrial wyosine in this lineage.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15031409
003      
CZ-PrNML
005      
20151014103220.0
007      
ta
008      
151005s2015 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/nar/gkv286 $2 doi
035    __
$a (PubMed)25845597
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Sample, Paul J $u Department of Microbiology and The Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.
245    12
$a A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria / $c PJ. Sample, L. Kořený, Z. Paris, KW. Gaston, MA. Rubio, IM. Fleming, S. Hinger, E. Horáková, PA. Limbach, J. Lukeš, JD. Alfonzo,
520    9_
$a Establishment of the early genetic code likely required strategies to ensure translational accuracy and inevitably involved tRNA post-transcriptional modifications. One such modification, wybutosine/wyosine is crucial for translational fidelity in Archaea and Eukarya; yet it does not occur in Bacteria and has never been described in mitochondria. Here, we present genetic, molecular and mass spectromery data demonstrating the first example of wyosine in mitochondria, a situation thus far unique to kinetoplastids. We also show that these modifications are important for mitochondrial function, underscoring their biological significance. This work focuses on TyW1, the enzyme required for the most critical step of wyosine biosynthesis. Based on molecular phylogeny, we suggest that the kinetoplastids pathways evolved via gene duplication and acquisition of an FMN-binding domain now prevalent in TyW1 of most eukaryotes. These findings are discussed in the context of the extensive U-insertion RNA editing in trypanosome mitochondria, which may have provided selective pressure for maintenance of mitochondrial wyosine in this lineage.
650    _2
$a guanosin $x analogy a deriváty $x biosyntéza $x chemie $x metabolismus $7 D006151
650    _2
$a mitochondrie $x enzymologie $7 D008928
650    _2
$a protozoální proteiny $x genetika $x metabolismus $7 D015800
650    _2
$a posttranskripční úpravy RNA $7 D012323
650    _2
$a RNA transferová $x chemie $x metabolismus $7 D012343
650    _2
$a Trypanosoma brucei brucei $x enzymologie $x genetika $7 D014346
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kořený, Luděk $u Institute of Parasitology, Biology Centre and Faculty of Sciences, University of South Bohemia, 37005 České Budějovice (Budweis), Czech Republic.
700    1_
$a Paris, Zdeněk $u Department of Microbiology and The Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.
700    1_
$a Gaston, Kirk W $u Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, USA.
700    1_
$a Rubio, Mary Anne T $u Department of Microbiology and The Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.
700    1_
$a Fleming, Ian M C $u Department of Microbiology and The Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.
700    1_
$a Hinger, Scott $u Department of Microbiology and The Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.
700    1_
$a Horáková, Eva $u Institute of Parasitology, Biology Centre and Faculty of Sciences, University of South Bohemia, 37005 České Budějovice (Budweis), Czech Republic.
700    1_
$a Limbach, Patrick A $u Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, USA.
700    1_
$a Lukeš, Julius $u Institute of Parasitology, Biology Centre and Faculty of Sciences, University of South Bohemia, 37005 České Budějovice (Budweis), Czech Republic Canadian Institute For Advanced Research, Toronto, ON M5G 1Z8, Canada.
700    1_
$a Alfonzo, Juan D $u Department of Microbiology and The Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, USA Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, USA alfonzo.1@osu.edu. $7 gn_A_00004107
773    0_
$w MED00003554 $t Nucleic acids research $x 1362-4962 $g Roč. 43, č. 8 (2015), s. 4262-73
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25845597 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20151005 $b ABA008
991    __
$a 20151014103410 $b ABA008
999    __
$a ok $b bmc $g 1092285 $s 914535
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 43 $c 8 $d 4262-73 $e 20150406 $i 1362-4962 $m Nucleic acids research $n Nucleic Acids Res $x MED00003554
LZP    __
$a Pubmed-20151005

Najít záznam

Citační ukazatele

Možnosti archivace