-
Something wrong with this record ?
Identification of individual biofilm-forming bacterial cells using Raman tweezers
O. Samek, S. Bernatová, J. Ježek, M. Šiler, M. Šerý, V. Krzyžánek, K. Hrubanová, P. Zemánek, V. Holá, F. Růžička,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NT13242
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
PubMed Central
from 2009
Europe PubMed Central
from 2009 to 1 year ago
ROAD: Directory of Open Access Scholarly Resources
from 1998
- MeSH
- Algorithms MeSH
- Principal Component Analysis MeSH
- Bacteria metabolism MeSH
- Biofilms * MeSH
- Cell Adhesion MeSH
- Phagocytosis MeSH
- Bacterial Physiological Phenomena MeSH
- Optical Tweezers * MeSH
- Polysaccharides chemistry MeSH
- Spectrum Analysis, Raman * MeSH
- Staphylococcus epidermidis metabolism MeSH
- Hot Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral “Raman fingerprints” obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilm-negative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15031444
- 003
- CZ-PrNML
- 005
- 20190702112752.0
- 007
- ta
- 008
- 151005s2015 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1117/1.JBO.20.5.051038 $2 doi
- 035 __
- $a (PubMed)25734616
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Samek, Ota $u Institute of Scientific Instruments of the AS CR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic. $7 _AN070693
- 245 10
- $a Identification of individual biofilm-forming bacterial cells using Raman tweezers / $c O. Samek, S. Bernatová, J. Ježek, M. Šiler, M. Šerý, V. Krzyžánek, K. Hrubanová, P. Zemánek, V. Holá, F. Růžička,
- 520 9_
- $a A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral “Raman fingerprints” obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilm-negative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.
- 650 _2
- $a algoritmy $7 D000465
- 650 _2
- $a Bacteria $x metabolismus $7 D001419
- 650 _2
- $a fyziologie bakterií $7 D018407
- 650 12
- $a biofilmy $7 D018441
- 650 _2
- $a buněčná adheze $7 D002448
- 650 _2
- $a vysoká teplota $7 D006358
- 650 12
- $a optická pinzeta $7 D052898
- 650 _2
- $a fagocytóza $7 D010587
- 650 _2
- $a polysacharidy $x chemie $7 D011134
- 650 _2
- $a analýza hlavních komponent $7 D025341
- 650 12
- $a Ramanova spektroskopie $7 D013059
- 650 _2
- $a Staphylococcus epidermidis $x metabolismus $7 D013212
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Bernatová, Silvie $u Institute of Scientific Instruments of the AS CR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
- 700 1_
- $a Ježek, Jan $u Institute of Scientific Instruments of the AS CR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
- 700 1_
- $a Šiler, Martin $u Institute of Scientific Instruments of the AS CR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
- 700 1_
- $a Šerý, Mojmir $u Institute of Scientific Instruments of the AS CR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
- 700 1_
- $a Krzyžánek, Vladislav $u Institute of Scientific Instruments of the AS CR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
- 700 1_
- $a Hrubanová, Kamila $u Institute of Scientific Instruments of the AS CR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
- 700 1_
- $a Zemánek, Pavel $u Institute of Scientific Instruments of the AS CR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
- 700 1_
- $a Holá, Veronika, $u Masaryk University and St. Anne's Faculty Hospital, Department of Microbiology, Faculty of Medicine, Pekarská 53, 656 91 Brno, Czech Republic. $d 1978- $7 xx0071618
- 700 1_
- $a Růžička, Filip, $u Masaryk University and St. Anne's Faculty Hospital, Department of Microbiology, Faculty of Medicine, Pekarská 53, 656 91 Brno, Czech Republic. $d 1972- $7 xx0071619
- 773 0_
- $w MED00180283 $t Journal of biomedical optics $x 1560-2281 $g Roč. 20, č. 5 (2015), s. 051038
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25734616 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20151005 $b ABA008
- 991 __
- $a 20190702112941 $b ABA008
- 999 __
- $a ok $b bmc $g 1092320 $s 914570
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 20 $c 5 $d 051038 $i 1560-2281 $m Journal of biomedical optics $n J Biomed Opt $x MED00180283
- GRA __
- $a NT13242 $p MZ0
- LZP __
- $a Pubmed-20151005
