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COI barcode based species-specific primers for identification of five species of stored-product pests from genus Cryptolestes (Coleoptera: Laemophloeidae)

Z. Varadínová, YJ. Wang, Z. Kučerová, V. Stejskal, G. Opit, Y. Cao, FJ. Li, ZH. Li,

. 2015 ; 105 (2) : 202-9. [pub] 20150122

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15031495
E-zdroje Online Plný text

NLK ProQuest Central od 2001-02-01 do Před 1 rokem
Health & Medicine (ProQuest) od 2001-02-01 do Před 1 rokem

Odkazy

PubMed 25609404
DOI 10.1017/s0007485315000024
Knihovny.cz E-zdroje

Flat grain beetles of the genus Cryptolestes (Coleoptera: Laemophloeidae) are one of the economically most important stored-product pests which feed on many kinds of agricultural products, especially grains. Nine of more than 40 described Cryptolestes species are recognized as stored-product pests and two of the pest species have a cosmopolitan distribution. Given the rapid growth in global trade of food products, ecological barriers to the spread of pests are easily overcome. Therefore, development of reliable systems for routine quarantine inspection and early infestation detection is vital. In the present study, we established a new rapid and accurate cytochrome c oxidase subunit I-based system for molecular identification of five common stored-product Cryptolestes species, namely, Cryptolestes capensis, Cryptolestes ferrugineus, Cryptolestes pusilloides, Cryptolestes pusillus and Cryptolestes turcicus. Five species-specific primer pairs for traditional uniplex polymerase chain reaction assay are described and their specificity and sensitivity for the identification process is evaluated using larval samples of 12 different populations from three continents (Asia, Europe and North America).

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