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Monodisperse carboxyl-functionalized poly(ethylene glycol)-coated magnetic poly(glycidyl methacrylate) microspheres: application to the immunocapture of β-amyloid peptides
D. Horák, H. Hlídková, M. Hiraoui, M. Taverna, V. Proks, E. Mázl Chánová, C. Smadja, Z. Kučerová,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25142028
DOI
10.1002/mabi.201400249
Knihovny.cz E-zdroje
- MeSH
- adsorpce MeSH
- amyloidní beta-protein izolace a purifikace MeSH
- chromatografie afinitní MeSH
- elektroforéza kapilární MeSH
- imunoglobulin G metabolismus MeSH
- kyseliny karboxylové chemie MeSH
- kyseliny polymethakrylové chemie MeSH
- magnetické jevy MeSH
- mikrosféry * MeSH
- mikroskopie atomárních sil MeSH
- peptidové fragmenty izolace a purifikace MeSH
- polyethylenglykoly chemie MeSH
- skot MeSH
- termogravimetrie MeSH
- velikost částic MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Identification and evaluation of small changes in β-amyloid peptide (Aβ) levels in cerebrospinal fluid is of crucial importance for early detection of Alzheimer's disease. Microfluidic detection methods enable effective preconcentration of Aβ using magnetic microparticles coated with Aβ antibodies. Poly(glycidyl methacrylate) microspheres are coated with α-amino-ω-methoxy-PEG5000 /α-amino-ω-Boc-NH-PEG5000 Boc groups deprotected and NH2 succinylated to introduce carboxyl groups. Capillary electrophoresis with laser-induced fluorescence detection confirms the efficient capture of Aβ 1-40 peptides on the microspheres with immobilized monoclonal anti-Aβ 6E10. The capture specificity is confirmed by comparing Aβ 1-40 levels on the anti-IgG-immobilized particles used as a control.
Citace poskytuje Crossref.org
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