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Isolation and characterization of glycocalyx in Streptomyces aureofaciens
J. Shabalin, I. Benes, Z. Hostálek, I. Kulayev,
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články
PubMed
1821871
Knihovny.cz E-zdroje
- MeSH
- aminokyseliny analýza MeSH
- frakcionace buněk MeSH
- glykoproteiny chemie izolace a purifikace MeSH
- polysacharidy chemie izolace a purifikace MeSH
- Streptomyces aureofaciens chemie MeSH
- Publikační typ
- časopisecké články MeSH
The surface layer, considered to be glycocalyx according to electron-microscopic observations, was separated from a low-production strain of Streptomyces aureofaciens by solubilization with urea and subsequent sonication. The isolation procedure was developed using various agents; neutral phosphatase served as a marker indicating the amount of the material released. The peripheral structure consisted predominantly of glycoprotein and differed from S-layers.
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- $a The surface layer, considered to be glycocalyx according to electron-microscopic observations, was separated from a low-production strain of Streptomyces aureofaciens by solubilization with urea and subsequent sonication. The isolation procedure was developed using various agents; neutral phosphatase served as a marker indicating the amount of the material released. The peripheral structure consisted predominantly of glycoprotein and differed from S-layers.
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