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MicroRNA profile in site-specific head and neck squamous cell cancer
D. Kalfert, M. Pesta, V. Kulda, O. Topolcan, A. Ryska, P. Celakovsky, J. Laco, M. Ludvikova,
Jazyk angličtina Země Řecko
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 2004 do Před 2 roky
Open Access Digital Library
od 2004-01-01
PubMed
25862914
Knihovny.cz E-zdroje
- MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA biosyntéza genetika MeSH
- nádorové biomarkery biosyntéza genetika MeSH
- nádory hlavy a krku genetika patologie MeSH
- nádory hrtanu genetika patologie MeSH
- nádory orofaryngu genetika patologie MeSH
- proliferace buněk genetika MeSH
- regulace genové exprese u nádorů MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- spinocelulární karcinom genetika patologie MeSH
- transkriptom genetika MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND/AIM: MicroRNAs (miRs) are non-coding RNA molecules regulating diverse cellular processes essential in carcinogenesis. Little is known regarding miRs in head and neck squamous cell cancer (HNSCC). The aim of the present study was to investigate miRs in relation to the clinico pathological features of site-specific HNSCC. MATERIALS AND METHODS: The study comprised of 51 patients with HNSCC (23 oropharyngeal, 24 laryngeal and 4 hypopharyngeal carcinomas). Total RNA was extracted from tumor tissue and normal squamous epithelium using the miRNeasy FFPE Kit. A quantitative estimation of let-7a, miR-21, miR-200c, miR-34a, miR-375 was performed by a real-time polymerase chain reaction (PCR) method using the TagMan® MicroRNA assay. Additionally, p16 expression was detected by immuno histo chemistry. RESULTS: Significant differences of let-7a, miR-200c, miR-34a levels between oropharyngeal and laryngeal cancers were found (p<0.05). Compared to non-neoplastic tissues, miR-21, miR-200c, miR-34a were up-regulated and miR-375 was down-regulated in tumors of all sites. MiR-34a tumor levels significantly correlated with oropharyngeal origin (p=0.0284) and p16 positivity (p=0.0218). CONCLUSION: The microRNA profile seems to play a potential role in the pathobiology of oropharyngeal and laryngeal HNSCC. Up-regulation of miR34a in p16-positive oropharyngeal cancer has not been so far described and additional studies are warranted.
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- $a Kalfert, David $u Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.
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- $a BACKGROUND/AIM: MicroRNAs (miRs) are non-coding RNA molecules regulating diverse cellular processes essential in carcinogenesis. Little is known regarding miRs in head and neck squamous cell cancer (HNSCC). The aim of the present study was to investigate miRs in relation to the clinico pathological features of site-specific HNSCC. MATERIALS AND METHODS: The study comprised of 51 patients with HNSCC (23 oropharyngeal, 24 laryngeal and 4 hypopharyngeal carcinomas). Total RNA was extracted from tumor tissue and normal squamous epithelium using the miRNeasy FFPE Kit. A quantitative estimation of let-7a, miR-21, miR-200c, miR-34a, miR-375 was performed by a real-time polymerase chain reaction (PCR) method using the TagMan® MicroRNA assay. Additionally, p16 expression was detected by immuno histo chemistry. RESULTS: Significant differences of let-7a, miR-200c, miR-34a levels between oropharyngeal and laryngeal cancers were found (p<0.05). Compared to non-neoplastic tissues, miR-21, miR-200c, miR-34a were up-regulated and miR-375 was down-regulated in tumors of all sites. MiR-34a tumor levels significantly correlated with oropharyngeal origin (p=0.0284) and p16 positivity (p=0.0218). CONCLUSION: The microRNA profile seems to play a potential role in the pathobiology of oropharyngeal and laryngeal HNSCC. Up-regulation of miR34a in p16-positive oropharyngeal cancer has not been so far described and additional studies are warranted.
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- $a Pesta, Martin $u Institute of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic Central Immunoanalytical Laboratory, Department of Nuclear Medicine, Faculty Hospital in Pilsen, Pilsen, Czech Republic.
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- $a Kulda, Vlastimil $u Department of Biochemistry, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic.
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- $a Topolcan, Ondrej $u Central Immunoanalytical Laboratory, Department of Nuclear Medicine, Faculty Hospital in Pilsen, Pilsen, Czech Republic.
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- $a Ryska, Ales $u The Fingerland Department of Pathology, University Hospital Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.
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- $a Celakovsky, Petr $u Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.
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- $a Laco, Jan $u The Fingerland Department of Pathology, University Hospital Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.
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- $a Ludvikova, Marie $u Institute of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic ludvikova.m@email.cz marie.ludvikova@lfp.cuni.cz.
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