• Something wrong with this record ?

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

D. Wrobel, D. Appelhans, M. Signorelli, B. Wiesner, D. Fessas, U. Scheler, B. Voit, J. Maly,

. 2015 ; 1848 (7) : 1490-501. (Complete edition) [pub] 20150403

Language English Country Netherlands

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc16000180

The influence of maltose-modified poly(propylene imine) (PPI) dendrimers on dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (DMPC/DMPG) (3%) liposomes was studied. Fourth generation (G4) PPI dendrimers with primary amino surface groups were partially (open shell glycodendrimers - OS) or completely (dense shell glycodendrimers - DS) modified with maltose residues. As a model membrane, two types of 100nm diameter liposomes were used to observe differences in the interactions between neutral DMPC and negatively charged DMPC/DMPG bilayers. Interactions were studied using fluorescence spectroscopy to evaluate the membrane fluidity of both the hydrophobic and hydrophilic parts of the lipid bilayer and using differential scanning calorimetry to investigate thermodynamic parameter changes. Pulsed-filed gradient NMR experiments were carried out to evaluate common diffusion coefficient of DMPG and DS PPI in D2O when using below critical micelle concentration of DMPG. Both OS and DS PPI G4 dendrimers show interactions with liposomes. Neutral DS dendrimers exhibit stronger changes in membrane fluidity compared to OS dendrimers. The bilayer structure seems more rigid in the case of anionic DMPC/DMPG liposomes in comparison to pure and neutral DMPC liposomes. Generally, interactions of dendrimers with anionic DMPC/DMPG and neutral DMPC liposomes were at the same level. Higher concentrations of positively charged OS dendrimers induced the aggregation process with negatively charged liposomes. For all types of experiments, the presence of NaCl decreased the strength of the interactions between glycodendrimers and liposomes. Based on NMR diffusion experiments we suggest that apart from electrostatic interactions for OS PPI hydrogen bonds play a major role in maltose-modified PPI dendrimer interactions with anionic and neutral model membranes where a contact surface is needed for undergoing multiple H-bond interactions between maltose shell of glycodendrimers and surface membrane of liposome.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16000180
003      
CZ-PrNML
005      
20160122104440.0
007      
ta
008      
160108s2015 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.bbamem.2015.03.033 $2 doi
035    __
$a (PubMed)25843678
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Wrobel, Dominika $u Department of Biology, Jan Evangelista Purkinje University, Usti nad Labem, Czech Republic. Electronic address: dominika.wrobel@ujep.cz.
245    10
$a Interaction study between maltose-modified PPI dendrimers and lipidic model membranes / $c D. Wrobel, D. Appelhans, M. Signorelli, B. Wiesner, D. Fessas, U. Scheler, B. Voit, J. Maly,
520    9_
$a The influence of maltose-modified poly(propylene imine) (PPI) dendrimers on dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (DMPC/DMPG) (3%) liposomes was studied. Fourth generation (G4) PPI dendrimers with primary amino surface groups were partially (open shell glycodendrimers - OS) or completely (dense shell glycodendrimers - DS) modified with maltose residues. As a model membrane, two types of 100nm diameter liposomes were used to observe differences in the interactions between neutral DMPC and negatively charged DMPC/DMPG bilayers. Interactions were studied using fluorescence spectroscopy to evaluate the membrane fluidity of both the hydrophobic and hydrophilic parts of the lipid bilayer and using differential scanning calorimetry to investigate thermodynamic parameter changes. Pulsed-filed gradient NMR experiments were carried out to evaluate common diffusion coefficient of DMPG and DS PPI in D2O when using below critical micelle concentration of DMPG. Both OS and DS PPI G4 dendrimers show interactions with liposomes. Neutral DS dendrimers exhibit stronger changes in membrane fluidity compared to OS dendrimers. The bilayer structure seems more rigid in the case of anionic DMPC/DMPG liposomes in comparison to pure and neutral DMPC liposomes. Generally, interactions of dendrimers with anionic DMPC/DMPG and neutral DMPC liposomes were at the same level. Higher concentrations of positively charged OS dendrimers induced the aggregation process with negatively charged liposomes. For all types of experiments, the presence of NaCl decreased the strength of the interactions between glycodendrimers and liposomes. Based on NMR diffusion experiments we suggest that apart from electrostatic interactions for OS PPI hydrogen bonds play a major role in maltose-modified PPI dendrimer interactions with anionic and neutral model membranes where a contact surface is needed for undergoing multiple H-bond interactions between maltose shell of glycodendrimers and surface membrane of liposome.
650    _2
$a diferenciální skenovací kalorimetrie $7 D002152
650    _2
$a dendrimery $x chemie $x metabolismus $7 D050091
650    _2
$a dimyristoylfosfatidylcholin $x chemie $x metabolismus $7 D004134
650    _2
$a difenylhexatrien $x chemie $7 D004161
650    _2
$a fluorescenční polarizace $7 D005454
650    _2
$a vodíková vazba $7 D006860
650    _2
$a hydrofobní a hydrofilní interakce $7 D057927
650    _2
$a lipidové dvojvrstvy $x chemie $x metabolismus $7 D008051
650    _2
$a liposomy $x chemie $x metabolismus $7 D008081
650    _2
$a magnetická rezonanční spektroskopie $7 D009682
650    _2
$a maltosa $x chemie $x metabolismus $7 D008320
650    _2
$a fluidita membrány $7 D008560
650    _2
$a membránové lipidy $x chemie $x metabolismus $7 D008563
650    _2
$a fosfatidylglyceroly $x chemie $x metabolismus $7 D010715
650    _2
$a polypropyleny $x chemie $x metabolismus $7 D011126
650    _2
$a statická elektřina $7 D055672
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Appelhans, Dietmar $u Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, 01069 Dresden, Germany. $7 gn_A_00007820
700    1_
$a Signorelli, Marco $u Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita di Milano, Milano, Italy.
700    1_
$a Wiesner, Brigitte $u Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, 01069 Dresden, Germany.
700    1_
$a Fessas, Dimitrios $u Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita di Milano, Milano, Italy.
700    1_
$a Scheler, Ulrich $u Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, 01069 Dresden, Germany.
700    1_
$a Voit, Brigitte $u Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, 01069 Dresden, Germany.
700    1_
$a Maly, Jan $u Department of Biology, Jan Evangelista Purkinje University, Usti nad Labem, Czech Republic.
773    0_
$w MED00009314 $t Biochimica et biophysica acta. Complete edition $x 0006-3002 $g Roč. 1848, č. 7 (2015), s. 1490-501
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25843678 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160108 $b ABA008
991    __
$a 20160122104600 $b ABA008
999    __
$a ok $b bmc $g 1102461 $s 924386
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 1848 $c 7 $d 1490-501 $e 20150403 $i 0006-3002 $m Biochimica et biophysica acta $n Biochim Biophys Acta $x MED00009314 $o Complete edition
LZP    __
$a Pubmed-20160108

Find record

Citation metrics

Archiving options