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Matrix-assisted laser desorption ionization-time of flight mass spectrometry meropenem hydrolysis assay with NH4HCO3, a reliable tool for direct detection of carbapenemase activity
CC. Papagiannitsis, V. Študentová, R. Izdebski, O. Oikonomou, Y. Pfeifer, E. Petinaki, J. Hrabák,
Language English Country United States
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1975 to 6 months ago
Freely Accessible Science Journals
from 1995 to 6 months ago
PubMed Central
from 1975 to 1 year ago
Europe PubMed Central
from 1975 to 6 months ago
Open Access Digital Library
from 1975-01-01
Open Access Digital Library
from 1975-01-01
PubMed
25694522
DOI
10.1128/jcm.03094-14
Knihovny.cz E-resources
- MeSH
- Electronic Data Processing MeSH
- Bacterial Proteins analysis MeSH
- beta-Lactamases analysis MeSH
- Bicarbonates * MeSH
- Hydrolysis MeSH
- Automation, Laboratory MeSH
- Humans MeSH
- Buffers MeSH
- Sensitivity and Specificity MeSH
- Software MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Thienamycins metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
A comparison of a matrix-assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH4HCO3 to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers.
Department of Microbiology Medical School University of Thessaly Biopolis Larissa Greece
National Medicines Institute Warsaw Poland
Robert Koch Institute Nosocomial Pathogens and Antibiotic Resistance Wernigerode Germany
References provided by Crossref.org
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