• Something wrong with this record ?

Elevated levels of 14-3-3 proteins, serotonin, gamma enolase and pyruvate kinase identified in clinical samples from patients diagnosed with colorectal cancer

P. Dowling, DJ. Hughes, AM. Larkin, J. Meiller, M. Henry, P. Meleady, V. Lynch, B. Pardini, A. Naccarati, M. Levy, P. Vodicka, P. Neary, M. Clynes,

. 2015 ; 441 (-) : 133-41. [pub] 20141223

Language English Country Netherlands

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc16000423

BACKGROUND: Colorectal cancer (CRC), a heterogeneous disease that is common in both men and women, continues to be one of the predominant cancers worldwide. Lifestyle, diet, environmental factors and gene defects all contribute towards CRC development risk. Therefore, the identification of novel biomarkers to aid in the management of CRC is crucial. The aim of the present study was to identify candidate biomarkers for CRC, and to develop a better understanding of their role in tumourogenesis. METHODS: In this study, both plasma and tissue samples from patients diagnosed with CRC, together with non-malignant and normal controls were examined using mass spectrometry based proteomics and metabolomics approaches. RESULTS: It was established that the level of several biomolecules, including serotonin, gamma enolase, pyruvate kinase and members of the 14-3-3 family of proteins, showed statistically significant changes when comparing malignant versus non-malignant patient samples, with a distinct pattern emerging mirroring cancer cell energy production. CONCLUSION: The diagnosis and management of CRC could be enhanced by the discovery and validation of new candidate biomarkers, as found in this study, aimed at facilitating early detection and/or patient stratification together with providing information on the complex behaviour of cancer cells.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16000423
003      
CZ-PrNML
005      
20160122123131.0
007      
ta
008      
160108s2015 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.cca.2014.12.005 $2 doi
035    __
$a (PubMed)25540887
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Dowling, Paul $u Department of Biology, National University of Ireland, Maynooth, Maynooth, Co. Kildare, Ireland; National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. Electronic address: paul.dowling@nuim.ie.
245    10
$a Elevated levels of 14-3-3 proteins, serotonin, gamma enolase and pyruvate kinase identified in clinical samples from patients diagnosed with colorectal cancer / $c P. Dowling, DJ. Hughes, AM. Larkin, J. Meiller, M. Henry, P. Meleady, V. Lynch, B. Pardini, A. Naccarati, M. Levy, P. Vodicka, P. Neary, M. Clynes,
520    9_
$a BACKGROUND: Colorectal cancer (CRC), a heterogeneous disease that is common in both men and women, continues to be one of the predominant cancers worldwide. Lifestyle, diet, environmental factors and gene defects all contribute towards CRC development risk. Therefore, the identification of novel biomarkers to aid in the management of CRC is crucial. The aim of the present study was to identify candidate biomarkers for CRC, and to develop a better understanding of their role in tumourogenesis. METHODS: In this study, both plasma and tissue samples from patients diagnosed with CRC, together with non-malignant and normal controls were examined using mass spectrometry based proteomics and metabolomics approaches. RESULTS: It was established that the level of several biomolecules, including serotonin, gamma enolase, pyruvate kinase and members of the 14-3-3 family of proteins, showed statistically significant changes when comparing malignant versus non-malignant patient samples, with a distinct pattern emerging mirroring cancer cell energy production. CONCLUSION: The diagnosis and management of CRC could be enhanced by the discovery and validation of new candidate biomarkers, as found in this study, aimed at facilitating early detection and/or patient stratification together with providing information on the complex behaviour of cancer cells.
650    _2
$a proteiny 14-3-3 $x krev $x metabolismus $7 D048948
650    _2
$a senioři $7 D000368
650    _2
$a kolorektální nádory $x krev $x diagnóza $7 D015179
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a hmotnostní spektrometrie $7 D013058
650    _2
$a metabolomika $7 D055432
650    _2
$a lidé středního věku $7 D008875
650    _2
$a fosfopyruváthydratasa $x krev $x metabolismus $7 D010751
650    _2
$a proteomika $7 D040901
650    _2
$a pyruvátkinasa $x krev $x metabolismus $7 D011770
650    _2
$a serotonin $x krev $x metabolismus $7 D012701
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Hughes, David J $u Department of Physiology and Medical Physics and Centre for Systems Medicine, Royal College of Surgeons in Ireland, Dublin 2, Ireland.
700    1_
$a Larkin, Anne Marie $u National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
700    1_
$a Meiller, Justine $u National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
700    1_
$a Henry, Michael $u National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
700    1_
$a Meleady, Paula $u National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
700    1_
$a Lynch, Vincent $u National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
700    1_
$a Pardini, Barbara $u Human Genetics Foundation, Turin, Italy.
700    1_
$a Naccarati, Alessio $u Human Genetics Foundation, Turin, Italy.
700    1_
$a Levy, Miroslav $u 1st Medical Faculty of Charles University and Thomayer University Hospital, Prague, Czech Republic.
700    1_
$a Vodicka, Pavel $u Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic; Institute of Biology and Medical Genetics, 1st Medical Faculty, Charles University, Prage, Czech Republic.
700    1_
$a Neary, Paul $u Department of Colorectal Surgery, AMNCH Hospital, Dublin 24, Ireland.
700    1_
$a Clynes, Martin $u National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
773    0_
$w MED00009464 $t Clinica chimica acta; international journal of clinical chemistry $x 1873-3492 $g Roč. 441, č. - (2015), s. 133-41
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25540887 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160108 $b ABA008
991    __
$a 20160122123251 $b ABA008
999    __
$a ok $b bmc $g 1102704 $s 924629
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 441 $c - $d 133-41 $e 20141223 $i 1873-3492 $m Clinica chimica acta $n Clin Chim Acta $x MED00009464
LZP    __
$a Pubmed-20160108

Find record

Citation metrics

Archiving options