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Synthesis and biological evaluation of nandrolone-bodipy conjugates
M. Jurášek, S. Rimpelová, V. Pavlíčková, T. Ruml, O. Lapčík, PB. Drašar,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Perzistentní odkaz
https://www.medvik.cz/link/bmc16000507
- MeSH
- fluorescenční barviva chemická syntéza chemie MeSH
- fluorescenční mikroskopie MeSH
- HEK293 buňky MeSH
- HeLa buňky MeSH
- lidé MeSH
- MFC-7 buňky MeSH
- molekulární konformace MeSH
- nádorové buněčné linie MeSH
- nandrolon chemie farmakologie MeSH
- proliferace buněk účinky léků MeSH
- sloučeniny boru chemie farmakologie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Here, we report synthesis and biological evaluation of fluorescent nandrolone-3-carboxymethyloxime derivatives conjugated with green-emitting bodipy dye via PEG linkers. All the newly-synthesized compounds were evaluated for their effect on cell proliferation in vitro in MCF-7, LNCaP, PC-3 and HEK 293T model cell lines using WST-1 assay. By means of live-cell fluorescence microscopy, the intracellular localization of nandrolone-bodipy conjugates was revealed in endoplasmic reticulum. Moreover, we performed competitive localization study with nonfluorescent nandrolone, metandrolone, boldenone, trenbolone, and testosterone.
Citace poskytuje Crossref.org
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- $a Jurášek, Michal $u Department of Chemistry of Natural Compounds, Institute of Chemical Technology in Prague, 166 28 Prague, Czech Republic.
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- $a Here, we report synthesis and biological evaluation of fluorescent nandrolone-3-carboxymethyloxime derivatives conjugated with green-emitting bodipy dye via PEG linkers. All the newly-synthesized compounds were evaluated for their effect on cell proliferation in vitro in MCF-7, LNCaP, PC-3 and HEK 293T model cell lines using WST-1 assay. By means of live-cell fluorescence microscopy, the intracellular localization of nandrolone-bodipy conjugates was revealed in endoplasmic reticulum. Moreover, we performed competitive localization study with nonfluorescent nandrolone, metandrolone, boldenone, trenbolone, and testosterone.
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- $a Rimpelová, Silvie $u Department of Biochemistry and Microbiology, Institute of Chemical Technology in Prague, 166 28 Prague, Czech Republic.
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- $a Pavlíčková, Vladimíra $u Department of Biochemistry and Microbiology, Institute of Chemical Technology in Prague, 166 28 Prague, Czech Republic.
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