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Induction and characterization of a replication competent cervid endogenous gammaretrovirus (CrERV) from mule deer cells
H. Fábryová, T. Hron, H. Kabíčková, M. Poss, D. Elleder,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biological Evolution MeSH
- Endogenous Retroviruses classification genetics isolation & purification ultrastructure MeSH
- Epithelial Cells ultrastructure virology MeSH
- Phylogeny MeSH
- Gammaretrovirus classification genetics isolation & purification ultrastructure MeSH
- Genome, Viral * MeSH
- HEK293 Cells MeSH
- Coculture Techniques MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Virus Replication MeSH
- Virion genetics isolation & purification ultrastructure MeSH
- Deer virology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Endogenous retroviruses (ERVs) were acquired during evolution of their host organisms after infection and mendelian inheritance in the germline by their exogenous counterparts. The ERVs can spread in the host genome and in some cases they affect the host phenotype. The cervid endogenous gammaretrovirus (CrERV) is one of only a few well-defined examples of evolutionarily recent invasion of mammalian genome by retroviruses. Thousands of insertionally polymorphic CrERV integration sites have been detected in wild ranging mule deer (Odocoileus hemionus) host populations. Here, we describe for the first time induction of replication competent CrERV by cocultivation of deer and human cells. We characterize the physical properties and tropism of the induced virus. The genomic sequence of the induced virus is phylogenetically related to the evolutionarily young endogenous CrERVs described so far. We also describe the level of replication block of CrERV on deer cells and its capacity to establish superinfection interference.
References provided by Crossref.org
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