Endogenous retroviruses (ERVs) were acquired during evolution of their host organisms after infection and mendelian inheritance in the germline by their exogenous counterparts. The ERVs can spread in the host genome and in some cases they affect the host phenotype. The cervid endogenous gammaretrovirus (CrERV) is one of only a few well-defined examples of evolutionarily recent invasion of mammalian genome by retroviruses. Thousands of insertionally polymorphic CrERV integration sites have been detected in wild ranging mule deer (Odocoileus hemionus) host populations. Here, we describe for the first time induction of replication competent CrERV by cocultivation of deer and human cells. We characterize the physical properties and tropism of the induced virus. The genomic sequence of the induced virus is phylogenetically related to the evolutionarily young endogenous CrERVs described so far. We also describe the level of replication block of CrERV on deer cells and its capacity to establish superinfection interference.
- MeSH
- biologická evoluce MeSH
- endogenní retroviry klasifikace genetika izolace a purifikace ultrastruktura MeSH
- epitelové buňky ultrastruktura virologie MeSH
- fylogeneze MeSH
- Gammaretrovirus klasifikace genetika izolace a purifikace ultrastruktura MeSH
- genom virový * MeSH
- HEK293 buňky MeSH
- kokultivační techniky MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- replikace viru MeSH
- virion genetika izolace a purifikace ultrastruktura MeSH
- vysoká zvěř virologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
