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Superresolution live imaging of plant cells using structured illumination microscopy
G. Komis, M. Mistrik, O. Šamajová, M. Ovečka, J. Bartek, J. Šamaj,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 2006-06-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2013-03-01 do 2015-12-31
Health & Medicine (ProQuest)
od 2006-06-01 do Před 1 rokem
PubMed
26203822
DOI
10.1038/nprot.2015.083
Knihovny.cz E-zdroje
- MeSH
- Arabidopsis MeSH
- intravitální mikroskopie metody MeSH
- rostlinné buňky * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Although superresolution (SR) approaches have been routinely used for fixed or living material from other organisms, the use of time-lapse structured illumination microscopy (SIM) imaging in plant cells still remains under-developed. Here we describe a validated method for time-lapse SIM that focuses on cortical microtubules of different plant cell types. By using one of the existing commercially available SIM platforms, we provide a user-friendly and easy-to-follow protocol that may be widely applied to the imaging of plant cells. This protocol includes steps describing calibration of the microscope and channel alignment, generation of an experimental point spread function (PSF), preparation of appropriate observation chambers with available plant material, image acquisition, reconstruction and validation. This protocol can be carried out within two to three working days.
Citace poskytuje Crossref.org
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- $a Bartek, Jiri $u 1] Institute of Molecular and Translational Medicine, Palacký University, Olomouc, Czech Republic. [2] Genome Integrity Unit, Danish Cancer Society Research Center, Copenhagen, Denmark.
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