-
Something wrong with this record ?
Drop-Coating Deposition Raman (DCDR) Spectroscopy as a Tool for Membrane Interaction Studies: Liposome-Porphyrin Complex
E. Kočišová, M. Procházka, L. Vaculčiaková,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26163374
DOI
10.1366/14-07836
Knihovny.cz E-resources
- MeSH
- Liposomes chemistry metabolism MeSH
- Membranes, Artificial * MeSH
- Porphyrins chemistry metabolism MeSH
- Spectrum Analysis, Raman methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Drop-coating deposition Raman (DCDR) spectroscopy is based on the measurement of a sample that has been preconcentrated by being dried on a special hydrophobic plate. In addition to its higher sensitivity, the advantage of DCDR over the conventional Raman spectroscopy is the small sample volume needed, the lack of interference from solvents, and the capability of segregating any impurities present and separating components in more complex samples. In this study, DCDR spectroscopy was employed to investigate the complex of the cationic copper(II) 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (CuTMPyP) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes. Drop-coating deposition Raman spectra were treated using factor analysis (FA), which led to the following conclusions: (i) the distribution of CuTMPyP in the complex is not homogenous, (ii) the DCDR technique segregates complexed and noncomplexed parts of the sample, (iii) the spectral changes caused by the drying process and by the interaction of CuTMPyP with the DPPC liposomes can be distinguished, and (iv) the porphyrin molecules interacting with DPPC affect both the order-disorder properties of the lipid chains and the lipid head.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16010044
- 003
- CZ-PrNML
- 005
- 20160412105309.0
- 007
- ta
- 008
- 160408s2015 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1366/14-07836 $2 doi
- 024 7_
- $a 10.1366/14-07836 $2 doi
- 035 __
- $a (PubMed)26163374
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Kočišová, Eva $u Charles University, Faculty of Mathematics and Physics, Institute of Physics, Ke Karlovu 5, Prague 2, CZ-121 16, Czech Republic.
- 245 10
- $a Drop-Coating Deposition Raman (DCDR) Spectroscopy as a Tool for Membrane Interaction Studies: Liposome-Porphyrin Complex / $c E. Kočišová, M. Procházka, L. Vaculčiaková,
- 520 9_
- $a Drop-coating deposition Raman (DCDR) spectroscopy is based on the measurement of a sample that has been preconcentrated by being dried on a special hydrophobic plate. In addition to its higher sensitivity, the advantage of DCDR over the conventional Raman spectroscopy is the small sample volume needed, the lack of interference from solvents, and the capability of segregating any impurities present and separating components in more complex samples. In this study, DCDR spectroscopy was employed to investigate the complex of the cationic copper(II) 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (CuTMPyP) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes. Drop-coating deposition Raman spectra were treated using factor analysis (FA), which led to the following conclusions: (i) the distribution of CuTMPyP in the complex is not homogenous, (ii) the DCDR technique segregates complexed and noncomplexed parts of the sample, (iii) the spectral changes caused by the drying process and by the interaction of CuTMPyP with the DPPC liposomes can be distinguished, and (iv) the porphyrin molecules interacting with DPPC affect both the order-disorder properties of the lipid chains and the lipid head.
- 650 _2
- $a liposomy $x chemie $x metabolismus $7 D008081
- 650 12
- $a membrány umělé $7 D008567
- 650 _2
- $a porfyriny $x chemie $x metabolismus $7 D011166
- 650 _2
- $a Ramanova spektroskopie $x metody $7 D013059
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Procházka, Marek
- 700 1_
- $a Vaculčiaková, Lenka
- 773 0_
- $w MED00008276 $t Applied spectroscopy $x 1943-3530 $g Roč. 69, č. 8 (2015), s. 939-45
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26163374 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160408 $b ABA008
- 991 __
- $a 20160412105352 $b ABA008
- 999 __
- $a ok $b bmc $g 1113473 $s 934412
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 69 $c 8 $d 939-45 $e 20150701 $i 1943-3530 $m Applied spectroscopy $n Appl Spectrosc $x MED00008276
- LZP __
- $a Pubmed-20160408