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Soluble NSF attachment protein receptor molecular mimicry by a Legionella pneumophila Dot/Icm effector

NP. King, P. Newton, R. Schuelein, DL. Brown, M. Petru, V. Zarsky, P. Dolezal, L. Luo, A. Bugarcic, AC. Stanley, RZ. Murray, BM. Collins, RD. Teasdale, EL. Hartland, JL. Stow,

. 2015 ; 17 (6) : 767-84. [pub] 20150124

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc16010668

Upon infection, Legionella pneumophila uses the Dot/Icm type IV secretion system to translocate effector proteins from the Legionella-containing vacuole (LCV) into the host cell cytoplasm. The effectors target a wide array of host cellular processes that aid LCV biogenesis, including the manipulation of membrane trafficking. In this study, we used a hidden Markov model screen to identify two novel, non-eukaryotic soluble NSF attachment protein receptor (SNARE) homologs: the bacterial Legionella SNARE effector A (LseA) and viral SNARE homolog A proteins. We characterized LseA as a Dot/Icm effector of L. pneumophila, which has close homology to the Qc-SNARE subfamily. The lseA gene was present in multiple sequenced L. pneumophila strains including Corby and was well distributed among L. pneumophila clinical and environmental isolates. Employing a variety of biochemical, cell biological and microbiological techniques, we found that farnesylated LseA localized to membranes associated with the Golgi complex in mammalian cells and LseA interacted with a subset of Qa-, Qb- and R-SNAREs in host cells. Our results suggested that LseA acts as a SNARE protein and has the potential to regulate or mediate membrane fusion events in Golgi-associated pathways.

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$a Upon infection, Legionella pneumophila uses the Dot/Icm type IV secretion system to translocate effector proteins from the Legionella-containing vacuole (LCV) into the host cell cytoplasm. The effectors target a wide array of host cellular processes that aid LCV biogenesis, including the manipulation of membrane trafficking. In this study, we used a hidden Markov model screen to identify two novel, non-eukaryotic soluble NSF attachment protein receptor (SNARE) homologs: the bacterial Legionella SNARE effector A (LseA) and viral SNARE homolog A proteins. We characterized LseA as a Dot/Icm effector of L. pneumophila, which has close homology to the Qc-SNARE subfamily. The lseA gene was present in multiple sequenced L. pneumophila strains including Corby and was well distributed among L. pneumophila clinical and environmental isolates. Employing a variety of biochemical, cell biological and microbiological techniques, we found that farnesylated LseA localized to membranes associated with the Golgi complex in mammalian cells and LseA interacted with a subset of Qa-, Qb- and R-SNAREs in host cells. Our results suggested that LseA acts as a SNARE protein and has the potential to regulate or mediate membrane fusion events in Golgi-associated pathways.
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$a Newton, Patrice $u Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Vic., Australia.
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$a Schuelein, Ralf $u Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Vic., Australia.
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$a Brown, Darren L $u Institute for Molecular Bioscience, The University of Queensland, Brisbane, Qld., Australia.
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$a Petru, Marketa $u Department of Parasitology, Charles University in Prague, Czech Republic.
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$a Zarsky, Vojtech $u Department of Parasitology, Charles University in Prague, Czech Republic.
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$a Dolezal, Pavel $u Department of Parasitology, Charles University in Prague, Czech Republic.
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$a Luo, Lin $u Institute for Molecular Bioscience, The University of Queensland, Brisbane, Qld., Australia.
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$a Stanley, Amanda C $u Institute for Molecular Bioscience, The University of Queensland, Brisbane, Qld., Australia.
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$a Collins, Brett M $u Institute for Molecular Bioscience, The University of Queensland, Brisbane, Qld., Australia.
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$a Teasdale, Rohan D $u Institute for Molecular Bioscience, The University of Queensland, Brisbane, Qld., Australia.
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$a Hartland, Elizabeth L $u Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Vic., Australia.
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$a Stow, Jennifer L $u Institute for Molecular Bioscience, The University of Queensland, Brisbane, Qld., Australia.
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