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Determination of Total Glutathione in Dried Blood Spot Samples Using a High-Performance Liquid Chromatography
R. Kanďár, X. Štramová, P. Drábková, M. Brandtnerová,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adult MeSH
- Glutathione blood chemistry MeSH
- Middle Aged MeSH
- Humans MeSH
- Limit of Detection MeSH
- Linear Models MeSH
- Methanol MeSH
- Adolescent MeSH
- Young Adult MeSH
- Reproducibility of Results MeSH
- Dried Blood Spot Testing methods MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A method is described for the determination of total glutathione (TGSH) in dried blood spot (DBS) samples using high-performance liquid chromatography with fluorescence detection. Whole blood and DBS samples were obtained from a group of blood donors. After GSH reduction with dithiothreitol and protein precipitation with ethanol, the samples were derivatized with naphthalene-2,3-dicarboxaldehyde to form a very stable, highly fluorescent derivative. For the separation, a reversed phase HPLC method was used. The mixture of ethanol and deionized water (8 : 92, v/v) was used as a mobile phase. The analytical performance of this method was satisfactory: the intra- and interassay coefficients of variation were below 10%. Quantitative recoveries from spiked DBS samples were between 98.3 and 103.6%. The presented method is inexpensive and suitable for clinical testing purposes.
References provided by Crossref.org
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