-
Something wrong with this record ?
Adsorpce heptamerů s různou středovou sekvencí nukleotidů na rtuťové elektrodě
[Adsorption of heptamers with different central nucleotide sequence at the mercury electrode]
Iveta Třísková, Libuše Trnková
Language Czech Country Czech Republic
Document type Research Support, Non-U.S. Gov't
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Adsorption MeSH
- Chemistry Techniques, Analytical MeSH
- DNA * ultrastructure MeSH
- Electrochemistry MeSH
- DNA Fragmentation MeSH
- Molecular Conformation * MeSH
- Simplexvirus genetics MeSH
- Spectrum Analysis MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
The DNA fragment d(GCGAAGC), occurring in the initial replication stage of the phage ?X174, in herpes simplex virus, in the promoter region of the heat-shock gene of E. coli and in rRNA genes is still in the focus of biological and biochemical research. This shortest hairpin guided us to study the relationship between the structure and function of other heptamers with changing triplet sequence in the center of the ODN molecule (AAA, GGG, CCC). Replacement of the central part of ODN was chosen because it was initially expected that the double peak of the G oxidation signal is due to the difference in the positions of G in the stem and a loop of the initially investigated d (GCGAAGC) fragment. The experiments, however, did not confirm this assumption. In this work, we have focused on the study of the interaction of selected heptamers with the surface of the mercury in order to answer the question to what extent the A + C reduction peaks and double-peak G oxidation signals are able to reflect the structures of heptamers affected by different central tri¬nucleotide sequences. Guanine residues d(GCGGGGC) in the structure of the quadruplex are resistant to structural changes and the voltammetric responses are therefore mini¬mized. The interactions of quadruplexes with the surface of the electrode are also weaker than interactions of hairpin structures d(GCGAAGC), d(GCAAAGC) and the duplex structure d(GCCCCGC). The presented research has shown that a small change in the central sequence not only can alter the structure (tetramolecular quadruplex, hairpin, bimolecular duplex) but also influences the electrochemical and adsorption properties of these molecules on the charged interface. Although electrochemistry is able to detect the various structural layout of the studied heptamers, it is desirable to support the electrochemical conclusions with other methods, e.g., UV-VIS absorption spectra reflecting chiroptic properties of ODNs and separation methods, such as gel electrophoresis (PAGE).
Adsorption of heptamers with different central nucleotide sequence at the mercury electrode
Literatura
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16015174
- 003
- CZ-PrNML
- 005
- 20160628114936.0
- 007
- ta
- 008
- 160524s2016 xr d f 000 0|cze||
- 009
- AR
- 040 __
- $a ABA008 $d ABA008 $e AACR2 $b cze
- 041 0_
- $a cze $b eng
- 044 __
- $a xr
- 100 1_
- $a Třísková, Iveta $7 _AN087228 $u Ústav chemie, Přírodovědecká fakulta, Masarykova univerzita, Brno
- 245 10
- $a Adsorpce heptamerů s různou středovou sekvencí nukleotidů na rtuťové elektrodě / $c Iveta Třísková, Libuše Trnková
- 246 31
- $a Adsorption of heptamers with different central nucleotide sequence at the mercury electrode
- 504 __
- $a Literatura
- 520 9_
- $a The DNA fragment d(GCGAAGC), occurring in the initial replication stage of the phage ?X174, in herpes simplex virus, in the promoter region of the heat-shock gene of E. coli and in rRNA genes is still in the focus of biological and biochemical research. This shortest hairpin guided us to study the relationship between the structure and function of other heptamers with changing triplet sequence in the center of the ODN molecule (AAA, GGG, CCC). Replacement of the central part of ODN was chosen because it was initially expected that the double peak of the G oxidation signal is due to the difference in the positions of G in the stem and a loop of the initially investigated d (GCGAAGC) fragment. The experiments, however, did not confirm this assumption. In this work, we have focused on the study of the interaction of selected heptamers with the surface of the mercury in order to answer the question to what extent the A + C reduction peaks and double-peak G oxidation signals are able to reflect the structures of heptamers affected by different central tri¬nucleotide sequences. Guanine residues d(GCGGGGC) in the structure of the quadruplex are resistant to structural changes and the voltammetric responses are therefore mini¬mized. The interactions of quadruplexes with the surface of the electrode are also weaker than interactions of hairpin structures d(GCGAAGC), d(GCAAAGC) and the duplex structure d(GCCCCGC). The presented research has shown that a small change in the central sequence not only can alter the structure (tetramolecular quadruplex, hairpin, bimolecular duplex) but also influences the electrochemical and adsorption properties of these molecules on the charged interface. Although electrochemistry is able to detect the various structural layout of the studied heptamers, it is desirable to support the electrochemical conclusions with other methods, e.g., UV-VIS absorption spectra reflecting chiroptic properties of ODNs and separation methods, such as gel electrophoresis (PAGE).
- 650 12
- $a DNA $x ultrastruktura $7 D004247
- 650 12
- $a molekulární konformace $7 D008968
- 650 _2
- $a elektrochemie $7 D004563
- 650 _2
- $a adsorpce $7 D000327
- 650 _2
- $a spektrální analýza $7 D013057
- 650 _2
- $a 2D gelová elektroforéza $7 D015180
- 650 _2
- $a chemické techniky analytické $7 D002623
- 650 _2
- $a fragmentace DNA $7 D053938
- 650 _2
- $a Simplexvirus $x genetika $7 D018139
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Trnková, Libuše, $d 1947- $7 jn20001005289 $u Ústav chemie, Přírodovědecká fakulta, Masarykova univerzita, Brno
- 773 0_
- $t Chemické listy $x 0009-2770 $g Roč. 110, č. 3 (2016), s. 222-228 $w MED00011010
- 856 41
- $u http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/222/222 $y domovská stránka časopisu
- 910 __
- $a ABA008 $b B 1918 $c 395 $y 4 $z 0
- 990 __
- $a 20160523135344 $b ABA008
- 991 __
- $a 20160628115122 $b ABA008
- 999 __
- $a ok $b bmc $g 1125373 $s 939603
- BAS __
- $a 3
- BMC __
- $a 2016 $b 110 $c 3 $d 222-228 $i 0009-2770 $m Chemické listy $n Chem. Listy $x MED00011010
- LZP __
- $c NLK137 $d 20160628 $a NLK 2016-19/pk
