Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Preparation and evaluation of carriers for detection of cholinesterase inhibitors

D. Vetchý, V. Pitschmann, M. Vetchá, T. Kašparovský, L. Matějovský,

. 2015 ; 36 Suppl 1 (-) : 95-9.

Jazyk angličtina Země Švédsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16020024

OBJECTIVES: The aim of the study was to use methods of pharmaceutical technology, and prepare carriers in the form of pellets suitable as a filling of detection tubes for enzymatic detection of cholinesterase inhibitors. The enzymatic detection was based on enzymatic hydrolysis of acetylthiocholine iodide and the subsequent colour reaction of its hydrolysis product with Ellman's reagent. The suitable carriers should be in the form of white, regular and sufficiently mechanically resistant particles of about 1 mm allowing it to capture the enzyme during the impregnation process and ensuring its high activity for enzymatic detection. METHODS: Carriers consisting of microcrystalline cellulose, lactose, povidone, and sodium carboxymethyl cellulose were prepared using extrusion-spheronization method under three different drying conditions in either a hot air oven or a microwave oven. Subsequently, the carriers were impregnated with acetylcholinesterase and their size, shape, mechanical resistance, bulk, tapped and pycnometric density, Hausner ratio, intraparticular and total tapped porosity, and activity were measured and recorded. RESULTS: In this procedure, carriers with different physical parameters and different acetylcholinesterase activity were evaluated. It was found that higher acetylcholinesterase activity was associated not only with a higher intraparticular porosity but also with more regular particles characterized by high sphericity and low total tapped porosity. CONCLUSION: This unique finding is important for the preparation of detection tubes based on enzymatic detection which is still irreplaceable especially in the field of detection and analysis of super-toxic cholinesterase inhibitors.

000      
00000naa a2200000 a 4500
001      
bmc16020024
003      
CZ-PrNML
005      
20160722122652.0
007      
ta
008      
160722s2015 sw f 000 0|eng||
009      
AR
035    __
$a (PubMed)26757125
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sw
100    1_
$a Vetchý, David $u Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
245    10
$a Preparation and evaluation of carriers for detection of cholinesterase inhibitors / $c D. Vetchý, V. Pitschmann, M. Vetchá, T. Kašparovský, L. Matějovský,
520    9_
$a OBJECTIVES: The aim of the study was to use methods of pharmaceutical technology, and prepare carriers in the form of pellets suitable as a filling of detection tubes for enzymatic detection of cholinesterase inhibitors. The enzymatic detection was based on enzymatic hydrolysis of acetylthiocholine iodide and the subsequent colour reaction of its hydrolysis product with Ellman's reagent. The suitable carriers should be in the form of white, regular and sufficiently mechanically resistant particles of about 1 mm allowing it to capture the enzyme during the impregnation process and ensuring its high activity for enzymatic detection. METHODS: Carriers consisting of microcrystalline cellulose, lactose, povidone, and sodium carboxymethyl cellulose were prepared using extrusion-spheronization method under three different drying conditions in either a hot air oven or a microwave oven. Subsequently, the carriers were impregnated with acetylcholinesterase and their size, shape, mechanical resistance, bulk, tapped and pycnometric density, Hausner ratio, intraparticular and total tapped porosity, and activity were measured and recorded. RESULTS: In this procedure, carriers with different physical parameters and different acetylcholinesterase activity were evaluated. It was found that higher acetylcholinesterase activity was associated not only with a higher intraparticular porosity but also with more regular particles characterized by high sphericity and low total tapped porosity. CONCLUSION: This unique finding is important for the preparation of detection tubes based on enzymatic detection which is still irreplaceable especially in the field of detection and analysis of super-toxic cholinesterase inhibitors.
650    _2
$a acetylthiocholin $x analogy a deriváty $x metabolismus $7 D000122
650    _2
$a sodná sůl karboxymethylcelulosy $x analýza $7 D002266
650    _2
$a celulosa $x analýza $7 D002482
650    _2
$a cholinesterasové inhibitory $x metabolismus $7 D002800
650    _2
$a kyselina dithionitrobenzoová $7 D004228
650    _2
$a laktosa $x analýza $7 D007785
650    _2
$a testování materiálů $7 D008422
650    _2
$a poréznost $7 D016062
650    _2
$a povidon $x analýza $7 D011205
650    _2
$a sulfhydrylová reagencia $7 D013439
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Pitschmann, Vladimír $u Oritest Ltd., Praha, Czech Republic.
700    1_
$a Vetchá, Martina $u Pharmacy at Mendel Square, Brno, Czech Republic.
700    1_
$a Kašparovský, Tomáš $u Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
700    1_
$a Matějovský, Lukáš $u Faculty of Environmental Technology, University of Chemistry and Technology, Praha, Czech Republic.
773    0_
$w MED00168352 $t Neuro endocrinology letters $x 0172-780X $g Roč. 36 Suppl 1, č. - (2015), s. 95-9
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26757125 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160722 $b ABA008
991    __
$a 20160722122906 $b ABA008
999    __
$a ok $b bmc $g 1154694 $s 944552
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 36 Suppl 1 $c - $d 95-9 $i 0172-780X $m Neuro-endocrinology letters $n Neuro-endocrinol. lett. $x MED00168352
LZP    __
$a Pubmed-20160722

Najít záznam

Citační ukazatele

Možnosti archivace