Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Bifidobacterium longum CCM 7952 Promotes Epithelial Barrier Function and Prevents Acute DSS-Induced Colitis in Strictly Strain-Specific Manner

D. Srutkova, M. Schwarzer, T. Hudcovic, Z. Zakostelska, V. Drab, A. Spanova, B. Rittich, H. Kozakova, I. Schabussova,

. 2015 ; 10 (7) : e0134050. [pub] 20150728

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc16020472

BACKGROUND: Reduced microbial diversity has been associated with inflammatory bowel disease (IBD) and probiotic bacteria have been proposed for its prevention and/or treatment. Nevertheless, comparative studies of strains of the same subspecies for specific health benefits are scarce. Here we compared two Bifidobacterium longum ssp. longum strains for their capacity to prevent experimental colitis. METHODS: Immunomodulatory properties of nine probiotic bifidobacteria were assessed by stimulation of murine splenocytes. The immune responses to B. longum ssp. longum CCM 7952 (Bl 7952) and CCDM 372 (Bl 372) were further characterized by stimulation of bone marrow-derived dendritic cell, HEK293/TLR2 or HEK293/NOD2 cells. A mouse model of dextran sulphate sodium (DSS)-induced colitis was used to compare their beneficial effects in vivo. RESULTS: The nine bifidobacteria exhibited strain-specific abilities to induce cytokine production. Bl 372 induced higher levels of both pro- and anti-inflammatory cytokines in spleen and dendritic cell cultures compared to Bl 7952. Both strains engaged TLR2 and contain ligands for NOD2. In a mouse model of DSS-induced colitis, Bl 7952, but not Bl 372, reduced clinical symptoms and preserved expression of tight junction proteins. Importantly, Bl 7952 improved intestinal barrier function as demonstrated by reduced FITC-dextran levels in serum. CONCLUSIONS: We have shown that Bl 7952, but not Bl 372, protected mice from the development of experimental colitis. Our data suggest that although some immunomodulatory properties might be widespread among the genus Bifidobacterium, others may be rare and characteristic only for a specific strain. Therefore, careful selection might be crucial in providing beneficial outcome in clinical trials with probiotics in IBD.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16020472
003      
CZ-PrNML
005      
20170522122648.0
007      
ta
008      
160722s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0134050 $2 doi
024    7_
$a 10.1371/journal.pone.0134050 $2 doi
035    __
$a (PubMed)26218526
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Srutkova, Dagmar $u Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Novy Hradek, Czech Republic.
245    10
$a Bifidobacterium longum CCM 7952 Promotes Epithelial Barrier Function and Prevents Acute DSS-Induced Colitis in Strictly Strain-Specific Manner / $c D. Srutkova, M. Schwarzer, T. Hudcovic, Z. Zakostelska, V. Drab, A. Spanova, B. Rittich, H. Kozakova, I. Schabussova,
520    9_
$a BACKGROUND: Reduced microbial diversity has been associated with inflammatory bowel disease (IBD) and probiotic bacteria have been proposed for its prevention and/or treatment. Nevertheless, comparative studies of strains of the same subspecies for specific health benefits are scarce. Here we compared two Bifidobacterium longum ssp. longum strains for their capacity to prevent experimental colitis. METHODS: Immunomodulatory properties of nine probiotic bifidobacteria were assessed by stimulation of murine splenocytes. The immune responses to B. longum ssp. longum CCM 7952 (Bl 7952) and CCDM 372 (Bl 372) were further characterized by stimulation of bone marrow-derived dendritic cell, HEK293/TLR2 or HEK293/NOD2 cells. A mouse model of dextran sulphate sodium (DSS)-induced colitis was used to compare their beneficial effects in vivo. RESULTS: The nine bifidobacteria exhibited strain-specific abilities to induce cytokine production. Bl 372 induced higher levels of both pro- and anti-inflammatory cytokines in spleen and dendritic cell cultures compared to Bl 7952. Both strains engaged TLR2 and contain ligands for NOD2. In a mouse model of DSS-induced colitis, Bl 7952, but not Bl 372, reduced clinical symptoms and preserved expression of tight junction proteins. Importantly, Bl 7952 improved intestinal barrier function as demonstrated by reduced FITC-dextran levels in serum. CONCLUSIONS: We have shown that Bl 7952, but not Bl 372, protected mice from the development of experimental colitis. Our data suggest that although some immunomodulatory properties might be widespread among the genus Bifidobacterium, others may be rare and characteristic only for a specific strain. Therefore, careful selection might be crucial in providing beneficial outcome in clinical trials with probiotics in IBD.
650    _2
$a zvířata $7 D000818
650    _2
$a Bifidobacterium $x klasifikace $x fyziologie $7 D001644
650    _2
$a kolitida $x chemicky indukované $x prevence a kontrola $7 D003092
650    _2
$a dendritické buňky $x mikrobiologie $x patologie $7 D003713
650    _2
$a síran dextranu $x toxicita $7 D016264
650    12
$a modely nemocí na zvířatech $7 D004195
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a HEK293 buňky $7 D057809
650    _2
$a lidé $7 D006801
650    _2
$a imunoenzymatické techniky $7 D007124
650    _2
$a střeva $x mikrobiologie $x patofyziologie $7 D007422
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední BALB C $7 D008807
650    _2
$a signální adaptorový protein Nod2 $x genetika $x metabolismus $7 D053473
650    _2
$a probiotika $x farmakologie $7 D019936
650    _2
$a toll-like receptor 2 $x genetika $x metabolismus $7 D051195
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Schwarzer, Martin $u Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Novy Hradek, Czech Republic.
700    1_
$a Hudcovic, Tomas $u Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Novy Hradek, Czech Republic.
700    1_
$a Jirásková-Zákostelská, Zuzana $u Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Prague, Czech Republic. $7 xx0117342
700    1_
$a Drab, Vladimir $u Dairy Research Institute Ltd., Prague, Czech Republic.
700    1_
$a Spanova, Alena $u Brno University of Technology, Faculty of Chemistry, Brno, Czech Republic.
700    1_
$a Rittich, Bohuslav $u Brno University of Technology, Faculty of Chemistry, Brno, Czech Republic.
700    1_
$a Kozakova, Hana $u Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Novy Hradek, Czech Republic.
700    1_
$a Schabussova, Irma $u Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 10, č. 7 (2015), s. e0134050
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26218526 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160722 $b ABA008
991    __
$a 20170522123042 $b ABA008
999    __
$a ok $b bmc $g 1155142 $s 945000
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 10 $c 7 $d e0134050 $e 20150728 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$a Pubmed-20160722
Back

Find record

Citation metrics

Archiving options