-
Something wrong with this record ?
Nanostructured composite material graphite/TiO2 and its antibacterial activity under visible light irradiation
K. Dědková, J. Lang, K. Matějová, P. Peikertová, J. Holešinský, V. Vodárek, J. Kukutschová,
Language English Country Switzerland
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anti-Bacterial Agents chemistry pharmacology MeSH
- Bacteria drug effects radiation effects MeSH
- Graphite chemistry MeSH
- Humans MeSH
- Nanoparticles chemistry MeSH
- Light * MeSH
- Titanium chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The paper addresses laboratory preparation, characterization and in vitro evaluation of antibacterial activity of graphite/TiO2 nanocomposites. Composites graphite/TiO2 with various ratio of TiO2 nanoparticles (30wt.%, and 50wt.%) to graphite were prepared using a thermal hydrolysis of titanylsulfate in the presence of graphite particles, and subsequently dried at 80°C. X-ray powder diffraction, transmission electron microscopy and Raman microspectroscopy served as phase-analytical methods distinguishing anatase and rutile phases in the prepared composites. Scanning and transmission electron microscopy techniques were used for characterization of morphology of the prepared samples. A developed modification of the standard microdilution test was used for in vitro evaluation of daylight induced antibacterial activity, using four common human pathogenic bacterial strains (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis and Pseudomonas aeruginosa). Antibacterial activity of the graphite/TiO2 nanocomposites could be based mainly on photocatalytic reaction with subsequent potential interaction of reactive oxygen species with bacterial cells. During the antibacterial activity experiments, the graphite/TiO2 nanocomposites exhibited antibacterial activity, where differences in the onset of activity and activity against bacterial strains were observed. The highest antibacterial activity evaluated as minimum inhibitory concentration was observed against P. aeruginosa after 180min of irradiation.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16020629
- 003
- CZ-PrNML
- 005
- 20161025105736.0
- 007
- ta
- 008
- 160722s2015 sz f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jphotobiol.2015.06.018 $2 doi
- 024 7_
- $a 10.1016/j.jphotobiol.2015.06.018 $2 doi
- 035 __
- $a (PubMed)26114221
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Dědková, Kateřina $u Nanotechnology Centre, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava-Poruba, Czech Republic; Regional Materials Science and Technology Centre, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava-Poruba, Czech Republic. Electronic address: katerina.dedkova@vsb.cz.
- 245 10
- $a Nanostructured composite material graphite/TiO2 and its antibacterial activity under visible light irradiation / $c K. Dědková, J. Lang, K. Matějová, P. Peikertová, J. Holešinský, V. Vodárek, J. Kukutschová,
- 520 9_
- $a The paper addresses laboratory preparation, characterization and in vitro evaluation of antibacterial activity of graphite/TiO2 nanocomposites. Composites graphite/TiO2 with various ratio of TiO2 nanoparticles (30wt.%, and 50wt.%) to graphite were prepared using a thermal hydrolysis of titanylsulfate in the presence of graphite particles, and subsequently dried at 80°C. X-ray powder diffraction, transmission electron microscopy and Raman microspectroscopy served as phase-analytical methods distinguishing anatase and rutile phases in the prepared composites. Scanning and transmission electron microscopy techniques were used for characterization of morphology of the prepared samples. A developed modification of the standard microdilution test was used for in vitro evaluation of daylight induced antibacterial activity, using four common human pathogenic bacterial strains (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis and Pseudomonas aeruginosa). Antibacterial activity of the graphite/TiO2 nanocomposites could be based mainly on photocatalytic reaction with subsequent potential interaction of reactive oxygen species with bacterial cells. During the antibacterial activity experiments, the graphite/TiO2 nanocomposites exhibited antibacterial activity, where differences in the onset of activity and activity against bacterial strains were observed. The highest antibacterial activity evaluated as minimum inhibitory concentration was observed against P. aeruginosa after 180min of irradiation.
- 650 _2
- $a antibakteriální látky $x chemie $x farmakologie $7 D000900
- 650 _2
- $a Bacteria $x účinky léků $x účinky záření $7 D001419
- 650 _2
- $a grafit $x chemie $7 D006108
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a světlo $7 D008027
- 650 _2
- $a nanočástice $x chemie $7 D053758
- 650 _2
- $a titan $x chemie $7 D014025
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Lang, Jaroslav $u Nanotechnology Centre, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava-Poruba, Czech Republic.
- 700 1_
- $a Matějová, Kateřina $u Agel Laboratories a.s., Clinical Microbiology Laboratory, Zalužanského 1192/15, 703 84 Ostrava-Vítkovice, Czech Republic.
- 700 1_
- $a Peikertová, Pavlína $u Nanotechnology Centre, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava-Poruba, Czech Republic.
- 700 1_
- $a Holešinský, Jan $u Department of Material Engineering, Faculty of Metallurgy and Materials Engineering, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava-Poruba, Czech Republic.
- 700 1_
- $a Vodárek, Vlastimil $u Regional Materials Science and Technology Centre, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava-Poruba, Czech Republic; Department of Material Engineering, Faculty of Metallurgy and Materials Engineering, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava-Poruba, Czech Republic.
- 700 1_
- $a Kukutschová, Jana $u Nanotechnology Centre, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava-Poruba, Czech Republic; Regional Materials Science and Technology Centre, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava-Poruba, Czech Republic.
- 773 0_
- $w MED00006656 $t Journal of photochemistry and photobiology. B, Biology $x 1873-2682 $g Roč. 149, č. - (2015), s. 265-71
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26114221 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160722 $b ABA008
- 991 __
- $a 20161025110150 $b ABA008
- 999 __
- $a ok $b bmc $g 1155299 $s 945157
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 149 $c - $d 265-71 $e 20150619 $i 1873-2682 $m Journal of photochemistry and photobiology. B, Biology $n J Photochem Photobiol B $x MED00006656
- LZP __
- $a Pubmed-20160722
