-
Something wrong with this record ?
Shifts in metabolomic profiles of the parasitoid Nasonia vitripennis associated with elevated cold tolerance induced by the parasitoid's diapause, host diapause and host diet augmented with proline
Y. Li, L. Zhang, H. Chen, V. Koštál, P. Simek, M. Moos, DL. Denlinger,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
- MeSH
- Diapause, Insect physiology MeSH
- Diet MeSH
- Glycolysis MeSH
- Larva growth & development metabolism MeSH
- Metabolome MeSH
- Cold Temperature MeSH
- Proline administration & dosage MeSH
- Sarcophagidae metabolism parasitology MeSH
- Wasps growth & development metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
The ectoparasitoid wasp, Nasonia vitripennis can enhance its cold tolerance by exploiting a maternally-induced larval diapause. A simple manipulation of the fly host diapause status and supplementation of the host diet with proline also dramatically increase cold tolerance in the parasitoid. In this study, we used a metabolomics approach to define alterations in metabolite profiles of N. vitripennis caused by diapause in the parasitoid, diapause of the host, and augmentation of the host's diet with proline. Metabolic profiles of diapausing and nondiapausing parasitoid were significantly differentiated, with pronounced distinctions in levels of multiple cryoprotectants, amino acids, and carbohydrates. The dynamic nature of diapause was underscored by a shift in the wasp's metabolomic profile as the duration of diapause increased, a feature especially evident for increased concentrations of a suite of cryoprotectants. Metabolic pathways involved in amino acid and carbohydrate metabolism were distinctly enriched during diapause in the parasitoid. Host diapause status also elicited a pronounced effect on metabolic signatures of the parasitoid, noted by higher cryoprotectants and elevated compounds derived from glycolysis. Proline supplementation of the host diet did not translate directly into elevated proline in the parasitoid but resulted in an alteration in the abundance of many other metabolites, including elevated concentrations of essential amino acids, and reduction in metabolites linked to energy utilization, lipid and amino acid metabolism. Thus, the enhanced cold tolerance of N. vitripennis associated with proline augmentation of the host diet appears to be an indirect effect caused by the metabolic perturbations associated with diet supplementation.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16020757
- 003
- CZ-PrNML
- 005
- 20160728103728.0
- 007
- ta
- 008
- 160722s2015 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.ibmb.2015.05.012 $2 doi
- 024 7_
- $a 10.1016/j.ibmb.2015.05.012 $2 doi
- 035 __
- $a (PubMed)26005120
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Li, Yuyan $u Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Departments of Entomology and Evolution, Ecology, and Organismal Biology, Ohio State University, Columbus, OH 43210, USA.
- 245 10
- $a Shifts in metabolomic profiles of the parasitoid Nasonia vitripennis associated with elevated cold tolerance induced by the parasitoid's diapause, host diapause and host diet augmented with proline / $c Y. Li, L. Zhang, H. Chen, V. Koštál, P. Simek, M. Moos, DL. Denlinger,
- 520 9_
- $a The ectoparasitoid wasp, Nasonia vitripennis can enhance its cold tolerance by exploiting a maternally-induced larval diapause. A simple manipulation of the fly host diapause status and supplementation of the host diet with proline also dramatically increase cold tolerance in the parasitoid. In this study, we used a metabolomics approach to define alterations in metabolite profiles of N. vitripennis caused by diapause in the parasitoid, diapause of the host, and augmentation of the host's diet with proline. Metabolic profiles of diapausing and nondiapausing parasitoid were significantly differentiated, with pronounced distinctions in levels of multiple cryoprotectants, amino acids, and carbohydrates. The dynamic nature of diapause was underscored by a shift in the wasp's metabolomic profile as the duration of diapause increased, a feature especially evident for increased concentrations of a suite of cryoprotectants. Metabolic pathways involved in amino acid and carbohydrate metabolism were distinctly enriched during diapause in the parasitoid. Host diapause status also elicited a pronounced effect on metabolic signatures of the parasitoid, noted by higher cryoprotectants and elevated compounds derived from glycolysis. Proline supplementation of the host diet did not translate directly into elevated proline in the parasitoid but resulted in an alteration in the abundance of many other metabolites, including elevated concentrations of essential amino acids, and reduction in metabolites linked to energy utilization, lipid and amino acid metabolism. Thus, the enhanced cold tolerance of N. vitripennis associated with proline augmentation of the host diet appears to be an indirect effect caused by the metabolic perturbations associated with diet supplementation.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a nízká teplota $7 D003080
- 650 _2
- $a diapauza hmyzu $x fyziologie $7 D064666
- 650 _2
- $a dieta $7 D004032
- 650 _2
- $a glykolýza $7 D006019
- 650 _2
- $a larva $x růst a vývoj $x metabolismus $7 D007814
- 650 _2
- $a metabolom $7 D055442
- 650 _2
- $a prolin $x aplikace a dávkování $7 D011392
- 650 _2
- $a Sarcophagidae $x metabolismus $x parazitologie $7 D058493
- 650 _2
- $a sršňovití $x růst a vývoj $x metabolismus $7 D014863
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 655 _2
- $a Research Support, U.S. Gov't, Non-P.H.S. $7 D013486
- 700 1_
- $a Zhang, Lisheng $u Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
- 700 1_
- $a Chen, Hongyin $u Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: hongyinc@163.com.
- 700 1_
- $a Koštál, Vladimir $u Institute of Entomology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic.
- 700 1_
- $a Simek, Petr $u Institute of Entomology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic.
- 700 1_
- $a Moos, Martin $u Institute of Entomology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic.
- 700 1_
- $a Denlinger, David L $u Departments of Entomology and Evolution, Ecology, and Organismal Biology, Ohio State University, Columbus, OH 43210, USA. Electronic address: denlinger.1@osu.edu.
- 773 0_
- $w MED00004948 $t Insect biochemistry and molecular biology $x 1879-0240 $g Roč. 63, č. - (2015), s. 34-46
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26005120 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160722 $b ABA008
- 991 __
- $a 20160728103950 $b ABA008
- 999 __
- $a ok $b bmc $g 1155427 $s 945285
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 63 $c - $d 34-46 $e 20150522 $i 1879-0240 $m Insect biochemistry and molecular biology $n Insect Biochem Mol Biol $x MED00004948
- LZP __
- $a Pubmed-20160722
