• Je něco špatně v tomto záznamu ?

Proteasomal control of cytokinin synthesis protects Mycobacterium tuberculosis against nitric oxide

MI. Samanovic, S. Tu, O. Novák, LM. Iyer, FE. McAllister, L. Aravind, SP. Gygi, SR. Hubbard, M. Strnad, KH. Darwin,

. 2015 ; 57 (6) : 984-94. [pub] 20150226

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16020952

One of several roles of the Mycobacterium tuberculosis proteasome is to defend against host-produced nitric oxide (NO), a free radical that can damage numerous biological macromolecules. Mutations that inactivate proteasomal degradation in Mycobacterium tuberculosis result in bacteria that are hypersensitive to NO and attenuated for growth in vivo, but it was not known why. To elucidate the link between proteasome function, NO resistance, and pathogenesis, we screened for suppressors of NO hypersensitivity in a mycobacterial proteasome ATPase mutant and identified mutations in Rv1205. We determined that Rv1205 encodes a pupylated proteasome substrate. Rv1205 is a homolog of the plant enzyme LONELY GUY, which catalyzes the production of hormones called cytokinins. Remarkably, we report that an obligate human pathogen secretes several cytokinins. Finally, we determined that the Rv1205-dependent accumulation of cytokinin breakdown products is likely responsible for the sensitization of Mycobacterium tuberculosis proteasome-associated mutants to NO.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16020952
003      
CZ-PrNML
005      
20160729120158.0
007      
ta
008      
160722s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.molcel.2015.01.024 $2 doi
024    7_
$a 10.1016/j.molcel.2015.01.024 $2 doi
035    __
$a (PubMed)25728768
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Samanovic, Marie I $u Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA.
245    10
$a Proteasomal control of cytokinin synthesis protects Mycobacterium tuberculosis against nitric oxide / $c MI. Samanovic, S. Tu, O. Novák, LM. Iyer, FE. McAllister, L. Aravind, SP. Gygi, SR. Hubbard, M. Strnad, KH. Darwin,
520    9_
$a One of several roles of the Mycobacterium tuberculosis proteasome is to defend against host-produced nitric oxide (NO), a free radical that can damage numerous biological macromolecules. Mutations that inactivate proteasomal degradation in Mycobacterium tuberculosis result in bacteria that are hypersensitive to NO and attenuated for growth in vivo, but it was not known why. To elucidate the link between proteasome function, NO resistance, and pathogenesis, we screened for suppressors of NO hypersensitivity in a mycobacterial proteasome ATPase mutant and identified mutations in Rv1205. We determined that Rv1205 encodes a pupylated proteasome substrate. Rv1205 is a homolog of the plant enzyme LONELY GUY, which catalyzes the production of hormones called cytokinins. Remarkably, we report that an obligate human pathogen secretes several cytokinins. Finally, we determined that the Rv1205-dependent accumulation of cytokinin breakdown products is likely responsible for the sensitization of Mycobacterium tuberculosis proteasome-associated mutants to NO.
650    _2
$a aldehydy $x metabolismus $7 D000447
650    _2
$a aminohydrolasy $x genetika $x metabolismus $7 D000619
650    _2
$a zvířata $7 D000818
650    _2
$a proteiny huseníčku $x metabolismus $7 D029681
650    _2
$a bakteriální proteiny $x chemie $x metabolismus $7 D001426
650    _2
$a cytokininy $x biosyntéza $x metabolismus $7 D003583
650    _2
$a interakce hostitele a patogenu $7 D054884
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a mutace $7 D009154
650    _2
$a Mycobacterium tuberculosis $x účinky léků $x genetika $x metabolismus $x patogenita $7 D009169
650    _2
$a oxid dusnatý $x metabolismus $x farmakologie $7 D009569
650    _2
$a proteasomový endopeptidasový komplex $x metabolismus $7 D046988
650    _2
$a suprese genetická $7 D013489
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Tu, Shengjiang $u Howard Hughes Medical Institute, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA.
700    1_
$a Novák, Ondřej $u Department of Metabolomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, 78371 Olomouc, Czech Republic; Laboratory of Growth Regulators, Institute of Experimental Botany AS CR, 78371 Olomouc, Czech Republic.
700    1_
$a Iyer, Lakshminarayan M $u National Center for Biotechnology Information, National Library of Medicine, NIH, Bethesda, MD 20894, USA.
700    1_
$a McAllister, Fiona E $u Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
700    1_
$a Aravind, L $u National Center for Biotechnology Information, National Library of Medicine, NIH, Bethesda, MD 20894, USA. $7 gn_A_00008052
700    1_
$a Gygi, Steven P $u Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
700    1_
$a Hubbard, Stevan R $u Department of Biochemistry and Molecular Pharmacology, The Skirball Institute, New York University School of Medicine, New York, NY 10016, USA.
700    1_
$a Strnad, Miroslav $u Department of Metabolomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, 78371 Olomouc, Czech Republic; Laboratory of Growth Regulators, Institute of Experimental Botany AS CR, 78371 Olomouc, Czech Republic.
700    1_
$a Darwin, K Heran $u Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA. Electronic address: heran.darwin@med.nyu.edu.
773    0_
$w MED00011398 $t Molecular cell $x 1097-4164 $g Roč. 57, č. 6 (2015), s. 984-94
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25728768 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160722 $b ABA008
991    __
$a 20160729120421 $b ABA008
999    __
$a ok $b bmc $g 1155622 $s 945480
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 57 $c 6 $d 984-94 $e 20150226 $i 1097-4164 $m Molecular cell $n Mol Cell $x MED00011398
LZP    __
$a Pubmed-20160722

Najít záznam

Citační ukazatele

Možnosti archivace