• Something wrong with this record ?

In vivo monitoring of rat macrophages labeled with poly(l-lysine)-iron oxide nanoparticles

M. Babič, M. Schmiedtová, R. Poledne, V. Herynek, D. Horák,

. 2015 ; 103 (6) : 1141-8. (Applied biomaterials) [pub] 20141006

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc16021051

Coprecipitation of FeCl2 and FeCl3 with aqueous ammonia was used to prepare iron oxide nanoparticles dispersible in aqueous medium. Oxidation of the particles with sodium hypochlorite then yielded maghemite (γ-Fe2 O3 ) nanoparticles which were coated with two types of coating -d-mannose or poly(l-lysine) (PLL) as confirmed by FTIR analysis. The particles were <10 nm according to transmission electron microscopy. Their hydrodynamic particle size was ∼180 nm (by dynamic light scattering). The d-mannose-, PLL-coated, and neat γ-Fe2 O3 particles as well as commercial Resovist® were used to label rat macrophages. The viability and contrast properties of labeled macrophages were compared. PLL-coated γ-Fe2 O3 nanoparticles were found optimal. The labeled macrophages were injected to rats monitored in vivo by magnetic resonance imaging up to 48 h. Transport of macrophages labeled with PLL-γ-Fe2 O3 nanoparticles in rats was confirmed. Tracking of macrophages using the developed particles can be used for monitoring of inflammations and cell migration in cell therapy.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16021051
003      
CZ-PrNML
005      
20160801101552.0
007      
ta
008      
160722s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1002/jbm.b.33292 $2 doi
024    7_
$a 10.1002/jbm.b.33292 $2 doi
035    __
$a (PubMed)25283523
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Babič, Michal $u Institute of Macromolecular Chemistry, Department of Polymer Particles, Academy of Sciences of the Czech Republic, 162 06, Prague 6, Czech Republic.
245    10
$a In vivo monitoring of rat macrophages labeled with poly(l-lysine)-iron oxide nanoparticles / $c M. Babič, M. Schmiedtová, R. Poledne, V. Herynek, D. Horák,
520    9_
$a Coprecipitation of FeCl2 and FeCl3 with aqueous ammonia was used to prepare iron oxide nanoparticles dispersible in aqueous medium. Oxidation of the particles with sodium hypochlorite then yielded maghemite (γ-Fe2 O3 ) nanoparticles which were coated with two types of coating -d-mannose or poly(l-lysine) (PLL) as confirmed by FTIR analysis. The particles were <10 nm according to transmission electron microscopy. Their hydrodynamic particle size was ∼180 nm (by dynamic light scattering). The d-mannose-, PLL-coated, and neat γ-Fe2 O3 particles as well as commercial Resovist® were used to label rat macrophages. The viability and contrast properties of labeled macrophages were compared. PLL-coated γ-Fe2 O3 nanoparticles were found optimal. The labeled macrophages were injected to rats monitored in vivo by magnetic resonance imaging up to 48 h. Transport of macrophages labeled with PLL-γ-Fe2 O3 nanoparticles in rats was confirmed. Tracking of macrophages using the developed particles can be used for monitoring of inflammations and cell migration in cell therapy.
650    _2
$a zvířata $7 D000818
650    _2
$a buněčný tracking $x metody $7 D058948
650    12
$a kontrastní látky $x chemie $x farmakologie $7 D003287
650    12
$a železité sloučeniny $x chemie $x farmakologie $7 D005290
650    _2
$a makrofágy $x radiografie $7 D008264
650    _2
$a magnetická rezonanční tomografie $x metody $7 D008279
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a nanočástice $x chemie $7 D053758
650    _2
$a velikost částic $7 D010316
650    12
$a polylysin $x chemie $x farmakologie $7 D011107
650    _2
$a krysa rodu Rattus $7 D051381
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Schmiedtová, Martina $u Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, 140 21, Prague 4, Czech Republic.
700    1_
$a Poledne, Rudolf $u Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, 140 21, Prague 4, Czech Republic.
700    1_
$a Herynek, Vít $u Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, 140 21, Prague 4, Czech Republic. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídeňská, 1083, Czech Republic.
700    1_
$a Horák, Daniel $u Institute of Macromolecular Chemistry, Department of Polymer Particles, Academy of Sciences of the Czech Republic, 162 06, Prague 6, Czech Republic.
773    0_
$w MED00007497 $t Journal of biomedical materials research. Part B, Applied biomaterials. Applied biomaterials $x 1552-4981 $g Roč. 103, č. 6 (2015), s. 1141-8
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25283523 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160722 $b ABA008
991    __
$a 20160801101819 $b ABA008
999    __
$a ok $b bmc $g 1155721 $s 945579
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 103 $c 6 $d 1141-8 $e 20141006 $i 1552-4981 $m Journal of biomedical materials research. Part B, Applied biomaterials $n J Biomed Mater Res $x MED00007497 $o Applied biomaterials
LZP    __
$a Pubmed-20160722

Find record

Citation metrics

Archiving options