• Je něco špatně v tomto záznamu ?

XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima

JE. Green, M. Dalíková, K. Sahara, F. Marec, M. Akam,

. 2016 ; 11 (2) : e0150292. [pub] 20160226

Jazyk angličtina Země Spojené státy americké

Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16027630

We show that the geophilomorph centipede Strigamia maritima possesses an XX/XY system of sex chromosomes, with males being the heterogametic sex. This is, to our knowledge, the first report of sex chromosomes in any geophilomorph centipede. Using the recently assembled Strigamia genome sequence, we identified a set of scaffolds differentially represented in male and female DNA sequence. Using quantitative real-time PCR, we confirmed that three candidate X chromosome-derived scaffolds are present at approximately twice the copy number in females as in males. Furthermore, we confirmed that six candidate Y chromosome-derived scaffolds contain male-specific sequences. Finally, using this molecular information, we designed an X chromosome-specific DNA probe and performed fluorescent in situ hybridization against mitotic and meiotic chromosome spreads to identify the Strigamia XY sex-chromosome pair cytologically. We found that the X and Y chromosomes are recognizably different in size during the early pachytene stage of meiosis, and exhibit incomplete and delayed pairing.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16027630
003      
CZ-PrNML
005      
20161005132230.0
007      
ta
008      
161005s2016 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0150292 $2 doi
024    7_
$a 10.1371/journal.pone.0150292 $2 doi
035    __
$a (PubMed)26919730
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Green, Jack E $u Laboratory for Development and Evolution, Department of Zoology, Downing Street, Cambridge, United Kingdom.
245    10
$a XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima / $c JE. Green, M. Dalíková, K. Sahara, F. Marec, M. Akam,
520    9_
$a We show that the geophilomorph centipede Strigamia maritima possesses an XX/XY system of sex chromosomes, with males being the heterogametic sex. This is, to our knowledge, the first report of sex chromosomes in any geophilomorph centipede. Using the recently assembled Strigamia genome sequence, we identified a set of scaffolds differentially represented in male and female DNA sequence. Using quantitative real-time PCR, we confirmed that three candidate X chromosome-derived scaffolds are present at approximately twice the copy number in females as in males. Furthermore, we confirmed that six candidate Y chromosome-derived scaffolds contain male-specific sequences. Finally, using this molecular information, we designed an X chromosome-specific DNA probe and performed fluorescent in situ hybridization against mitotic and meiotic chromosome spreads to identify the Strigamia XY sex-chromosome pair cytologically. We found that the X and Y chromosomes are recognizably different in size during the early pachytene stage of meiosis, and exhibit incomplete and delayed pairing.
650    _2
$a zvířata $7 D000818
650    _2
$a členovci $x embryologie $x genetika $x růst a vývoj $x fyziologie $7 D001181
650    _2
$a srovnávací genomová hybridizace $7 D055028
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a hybridizace in situ fluorescenční $7 D017404
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a kvantitativní polymerázová řetězová reakce $7 D060888
650    _2
$a procesy určující pohlaví $x genetika $7 D019849
650    12
$a chromozom X $x ultrastruktura $7 D014960
650    12
$a chromozom Y $x ultrastruktura $7 D014998
655    _2
$a srovnávací studie $7 D003160
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Dalíková, Martina $u Laboratory of Molecular Cytogenetics, Institute of Entomology, Biology Centre CAS, Branišovská 31, 370 05 České Budějovice, Czech Republic.
700    1_
$a Sahara, Ken $u Laboratory of Applied Entomology, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.
700    1_
$a Marec, František $u Laboratory of Molecular Cytogenetics, Institute of Entomology, Biology Centre CAS, Branišovská 31, 370 05 České Budějovice, Czech Republic.
700    1_
$a Akam, Michael $u Laboratory for Development and Evolution, Department of Zoology, Downing Street, Cambridge, United Kingdom. $7 gn_A_00002810
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 11, č. 2 (2016), s. e0150292
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26919730 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20161005 $b ABA008
991    __
$a 20161005132617 $b ABA008
999    __
$a ok $b bmc $g 1165944 $s 952260
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 11 $c 2 $d e0150292 $e 20160226 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$a Pubmed-20161005

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...