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XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima
JE. Green, M. Dalíková, K. Sahara, F. Marec, M. Akam,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2006
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od 2006-10-01
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od 2008-01-01
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- MeSH
- chromozom X * ultrastruktura MeSH
- chromozom Y * ultrastruktura MeSH
- členovci embryologie genetika růst a vývoj fyziologie MeSH
- hybridizace in situ fluorescenční MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- procesy určující pohlaví genetika MeSH
- srovnávací genomová hybridizace MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
We show that the geophilomorph centipede Strigamia maritima possesses an XX/XY system of sex chromosomes, with males being the heterogametic sex. This is, to our knowledge, the first report of sex chromosomes in any geophilomorph centipede. Using the recently assembled Strigamia genome sequence, we identified a set of scaffolds differentially represented in male and female DNA sequence. Using quantitative real-time PCR, we confirmed that three candidate X chromosome-derived scaffolds are present at approximately twice the copy number in females as in males. Furthermore, we confirmed that six candidate Y chromosome-derived scaffolds contain male-specific sequences. Finally, using this molecular information, we designed an X chromosome-specific DNA probe and performed fluorescent in situ hybridization against mitotic and meiotic chromosome spreads to identify the Strigamia XY sex-chromosome pair cytologically. We found that the X and Y chromosomes are recognizably different in size during the early pachytene stage of meiosis, and exhibit incomplete and delayed pairing.
Citace poskytuje Crossref.org
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