Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Prolyl Isomerase Pin1 Regulates Axon Guidance by Stabilizing CRMP2A Selectively in Distal Axons

M. Balastik, XZ. Zhou, M. Alberich-Jorda, R. Weissova, J. Žiak, MF. Pazyra-Murphy, KE. Cosker, O. Machonova, I. Kozmikova, CH. Chen, L. Pastorino, JM. Asara, A. Cole, C. Sutherland, RA. Segal, KP. Lu,

. 2015 ; 13 (4) : 812-28. [pub] 20151017

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16028064

Axon guidance relies on precise translation of extracellular signal gradients into local changes in cytoskeletal dynamics, but the molecular mechanisms regulating dose-dependent responses of growth cones are still poorly understood. Here, we show that during embryonic development in growing axons, a low level of Semaphorin3A stimulation is buffered by the prolyl isomerase Pin1. We demonstrate that Pin1 stabilizes CDK5-phosphorylated CRMP2A, the major isoform of CRMP2 in distal axons. Consequently, Pin1 knockdown or knockout reduces CRMP2A levels specifically in distal axons and inhibits axon growth, which can be fully rescued by Pin1 or CRMP2A expression. Moreover, Pin1 knockdown or knockout increases sensitivity to Sema3A-induced growth cone collapse in vitro and in vivo, leading to developmental abnormalities in axon guidance. These results identify an important isoform-specific function and regulation of CRMP2A in controlling axon growth and uncover Pin1-catalyzed prolyl isomerization as a regulatory mechanism in axon guidance.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16028064
003      
CZ-PrNML
005      
20161021102854.0
007      
ta
008      
161005s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.celrep.2015.09.026 $2 doi
024    7_
$a 10.1016/j.celrep.2015.09.026 $2 doi
035    __
$a (PubMed)26489457
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Balastik, Martin $u Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 0408, Boston, MA 02215, USA; Institute of Molecular Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic; Institute of Physiology, Vídeňská 1083, 142 20 Prague 4, Czech Republic. Electronic address: martin.balastik@fgu.cas.cz.
245    10
$a Prolyl Isomerase Pin1 Regulates Axon Guidance by Stabilizing CRMP2A Selectively in Distal Axons / $c M. Balastik, XZ. Zhou, M. Alberich-Jorda, R. Weissova, J. Žiak, MF. Pazyra-Murphy, KE. Cosker, O. Machonova, I. Kozmikova, CH. Chen, L. Pastorino, JM. Asara, A. Cole, C. Sutherland, RA. Segal, KP. Lu,
520    9_
$a Axon guidance relies on precise translation of extracellular signal gradients into local changes in cytoskeletal dynamics, but the molecular mechanisms regulating dose-dependent responses of growth cones are still poorly understood. Here, we show that during embryonic development in growing axons, a low level of Semaphorin3A stimulation is buffered by the prolyl isomerase Pin1. We demonstrate that Pin1 stabilizes CDK5-phosphorylated CRMP2A, the major isoform of CRMP2 in distal axons. Consequently, Pin1 knockdown or knockout reduces CRMP2A levels specifically in distal axons and inhibits axon growth, which can be fully rescued by Pin1 or CRMP2A expression. Moreover, Pin1 knockdown or knockout increases sensitivity to Sema3A-induced growth cone collapse in vitro and in vivo, leading to developmental abnormalities in axon guidance. These results identify an important isoform-specific function and regulation of CRMP2A in controlling axon growth and uncover Pin1-catalyzed prolyl isomerization as a regulatory mechanism in axon guidance.
650    _2
$a zvířata $7 D000818
650    _2
$a axony $x metabolismus $7 D001369
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a imunohistochemie $7 D007150
650    _2
$a imunoprecipitace $7 D047468
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a proteiny nervové tkáně $x genetika $x metabolismus $7 D009419
650    _2
$a peptidylprolylisomerasa $x genetika $x metabolismus $7 D019696
650    _2
$a fosforylace $7 D010766
650    _2
$a signální transdukce $7 D015398
650    _2
$a dánio pruhované $7 D015027
650    _2
$a proteiny dánia pruhovaného $x genetika $x metabolismus $7 D029961
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Zhou, Xiao Zhen $u Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 0408, Boston, MA 02215, USA.
700    1_
$a Alberich-Jorda, Meritxell $u Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 0408, Boston, MA 02215, USA; Institute of Molecular Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic. $7 gn_A_00003411
700    1_
$a Weissova, Romana $u Institute of Molecular Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic; Institute of Physiology, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
700    1_
$a Žiak, Jakub $u Institute of Molecular Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic; Institute of Physiology, Vídeňská 1083, 142 20 Prague 4, Czech Republic. $7 xx0207153
700    1_
$a Pazyra-Murphy, Maria F $u Department of Pediatric Oncology and Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
700    1_
$a Cosker, Katharina E $u Department of Pediatric Oncology and Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
700    1_
$a Machonova, Olga $u Institute of Molecular Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
700    1_
$a Kozmikova, Iryna $u Institute of Molecular Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
700    1_
$a Chen, Chun-Hau $u Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 0408, Boston, MA 02215, USA.
700    1_
$a Pastorino, Lucia $u Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 0408, Boston, MA 02215, USA.
700    1_
$a Asara, John M $u Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 0408, Boston, MA 02215, USA. $7 gn_A_00009185
700    1_
$a Cole, Adam $u Biomedical Research Institute, University of Dundee, Ninewells Hospital, Dundee DD1 9SY, Scotland, UK.
700    1_
$a Sutherland, Calum $u Biomedical Research Institute, University of Dundee, Ninewells Hospital, Dundee DD1 9SY, Scotland, UK.
700    1_
$a Segal, Rosalind A $u Department of Pediatric Oncology and Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
700    1_
$a Lu, Kun Ping $u Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 0408, Boston, MA 02215, USA; Institute for Translational Medicine, Fujian Medical University, Fuzhou 350108, China. Electronic address: klu@bidmc.harvard.edu.
773    0_
$w MED00188029 $t Cell reports $x 2211-1247 $g Roč. 13, č. 4 (2015), s. 812-28
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26489457 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20161005 $b ABA008
991    __
$a 20161021103302 $b ABA008
999    __
$a ok $b bmc $g 1166378 $s 952694
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 13 $c 4 $d 812-28 $e 20151017 $i 2211-1247 $m Cell reports $n Cell Rep $x MED00188029
LZP    __
$a Pubmed-20161005

Najít záznam

Citační ukazatele

Možnosti archivace