-
Je něco špatně v tomto záznamu ?
Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases
A. Olszewska, J. Dadová, M. Mačková, M. Hocek,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- DNA analýza metabolismus MeSH
- flucytosin chemie MeSH
- fluoruracil chemie MeSH
- restrikční endonukleasy typu II metabolismus MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- štěpení DNA MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A systematic study of the cleavage of DNA sequences containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases (REs) was performed and the results compared with the same sequences containing natural pyrimidine bases, uracil or 5-methylcytosine. The results show that some REs recognize fluorine as a hydrogen on cytosine and cleave the corresponding sequences where the presence of m5dC leads to blocking of the cleavage. However, on uracil, the same REs recognize the F as a methyl surrogate and cleave the sequences which are not cleaved if uracil is incorporated instead of thymine. These results are interesting for understanding the recognition of DNA sequences by REs and for manipulation of the specific DNA cutting.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16028099
- 003
- CZ-PrNML
- 005
- 20231213112919.0
- 007
- ta
- 008
- 161005s2015 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.bmc.2015.09.051 $2 doi
- 024 7_
- $a 10.1016/j.bmc.2015.09.051 $2 doi
- 035 __
- $a (PubMed)26463367
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Olszewska, Agata $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, CZ-16610 Prague 6, Czech Republic.
- 245 10
- $a Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases / $c A. Olszewska, J. Dadová, M. Mačková, M. Hocek,
- 520 9_
- $a A systematic study of the cleavage of DNA sequences containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases (REs) was performed and the results compared with the same sequences containing natural pyrimidine bases, uracil or 5-methylcytosine. The results show that some REs recognize fluorine as a hydrogen on cytosine and cleave the corresponding sequences where the presence of m5dC leads to blocking of the cleavage. However, on uracil, the same REs recognize the F as a methyl surrogate and cleave the sequences which are not cleaved if uracil is incorporated instead of thymine. These results are interesting for understanding the recognition of DNA sequences by REs and for manipulation of the specific DNA cutting.
- 650 _2
- $a DNA $x analýza $x metabolismus $7 D004247
- 650 _2
- $a štěpení DNA $7 D053837
- 650 _2
- $a restrikční endonukleasy typu II $x metabolismus $7 D015252
- 650 _2
- $a flucytosin $x chemie $7 D005437
- 650 _2
- $a fluoruracil $x chemie $7 D005472
- 650 _2
- $a spektrometrie hmotnostní - ionizace laserem za účasti matrice $7 D019032
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Daďová, Jitka, $d 1986- $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, CZ-16610 Prague 6, Czech Republic. $7 xx0311498
- 700 1_
- $a Mačková, Michaela $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, CZ-16610 Prague 6, Czech Republic.
- 700 1_
- $a Hocek, Michal $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, CZ-16610 Prague 6, Czech Republic; Department of Organic and Nuclear Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-12843 Prague 2, Czech Republic. Electronic address: hocek@uochb.cas.cz.
- 773 0_
- $w MED00000769 $t Bioorganic & medicinal chemistry $x 1464-3391 $g Roč. 23, č. 21 (2015), s. 6885-90
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26463367 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20161005 $b ABA008
- 991 __
- $a 20231213112915 $b ABA008
- 999 __
- $a ok $b bmc $g 1166413 $s 952729
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 23 $c 21 $d 6885-90 $e 20151014 $i 1464-3391 $m Bioorganic & medicinal chemistry $n Bioorg Med Chem $x MED00000769
- LZP __
- $a Pubmed-20161005
