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A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction
EJ. Faassen, MG. Antoniou, W. Beekman-Lukassen, L. Blahova, E. Chernova, C. Christophoridis, A. Combes, C. Edwards, J. Fastner, J. Harmsen, A. Hiskia, LL. Ilag, T. Kaloudis, S. Lopicic, M. Lürling, H. Mazur-Marzec, J. Meriluoto, C. Porojan, Y....
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2003
Free Medical Journals
od 2003
PubMed Central
od 2003
Europe PubMed Central
od 2003
ProQuest Central
od 2003-01-01
Open Access Digital Library
od 2003-01-01
Open Access Digital Library
od 2003-01-01
Health & Medicine (ProQuest)
od 2003-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2003
PubMed
26938542
DOI
10.3390/md14030045
Knihovny.cz E-zdroje
- MeSH
- aminokyseliny diaminové analýza metabolismus MeSH
- chromatografie kapalinová metody MeSH
- Daphnia MeSH
- kyselina trichloroctová chemie MeSH
- mozek metabolismus MeSH
- neurotoxiny analýza metabolismus MeSH
- reprodukovatelnost výsledků MeSH
- sinice metabolismus MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Exposure to β-N-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D₃BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D₃BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (<10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis.
Alterra P O Box 47 Wageningen 6700 DD The Netherlands
D) Athens Water Supply and Sewerage Company 156 Oropou str 11146 Athens Greece
Faculty of Science RECETOX Masaryk University Kamenice 5 625 00 Brno Czech Republic
Kinneret Limnological Laboratory Israel Oceanographic and amp
Life Sciences Robert Gordon University Aberdeen AB10 7GJ UK
Limnological Research P O Box 447 Migdal 14950 Israel
Nanotechnology National Center for Scientific Research Demokritos Patriarchou Grigoriou and amp
Neapoleos 15310 Agia Paraskevi Athens Greece
Water Quality Department Division of Quality Research and Development (R and amp
Water Quality Management Group Wageningen University P O Box 47 Wageningen 6700 DD The Netherlands
Citace poskytuje Crossref.org
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- $a Faassen, Elisabeth J $u Aquatic Ecology & Water Quality Management Group, Wageningen University, P.O. Box 47, Wageningen 6700 DD, The Netherlands. els.faassen@wur.nl.
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- $a Exposure to β-N-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D₃BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D₃BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (<10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis.
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