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Fully automatic flow-based device for monitoring of drug permeation across a cell monolayer
L. Zelená, SS. Marques, MA. Segundo, M. Miró, P. Pávek, H. Sklenářová, P. Solich,
Jazyk angličtina Země Německo
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 2011-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2003-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2011-01-01 do Před 1 rokem
- MeSH
- automatizace metody MeSH
- biologický transport MeSH
- epitelové buňky chemie metabolismus MeSH
- kinetika MeSH
- lidé MeSH
- P-glykoprotein metabolismus MeSH
- průtoková injekční analýza přístrojové vybavení metody MeSH
- rhodamin 123 chemie metabolismus MeSH
- verapamil chemie metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
A novel flow-programming setup based on the sequential injection principle is herein proposed for on-line monitoring of temporal events in cell permeation studies. The permeation unit consists of a Franz cell with its basolateral compartment mixed under mechanical agitation and thermostated at 37 °C. The apical compartment is replaced by commercially available Transwell inserts with a precultivated cell monolayer. The transport of drug substances across epithelial cells genetically modified with the P-glycoprotein membrane transporter (MDCKII-MDR1) is monitored on-line using rhodamine 123 as a fluorescent marker. The permeation kinetics of the marker is obtained in a fully automated mode by sampling minute volumes of solution from the basolateral compartment in short intervals (10 min) up to 4 h. The effect of a P-glycoprotein transporter inhibitor, verapamil as a model drug, on the efficiency of the marker transport across the cell monolayer is thoroughly investigated. The analytical features of the proposed flow method for cell permeation studies in real time are critically compared against conventional batch-wise procedures and microfluidic devices.
Citace poskytuje Crossref.org
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- $a Zelená, Lucie $u Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, 500 05, Hradec Králové, Czech Republic.
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- $a A novel flow-programming setup based on the sequential injection principle is herein proposed for on-line monitoring of temporal events in cell permeation studies. The permeation unit consists of a Franz cell with its basolateral compartment mixed under mechanical agitation and thermostated at 37 °C. The apical compartment is replaced by commercially available Transwell inserts with a precultivated cell monolayer. The transport of drug substances across epithelial cells genetically modified with the P-glycoprotein membrane transporter (MDCKII-MDR1) is monitored on-line using rhodamine 123 as a fluorescent marker. The permeation kinetics of the marker is obtained in a fully automated mode by sampling minute volumes of solution from the basolateral compartment in short intervals (10 min) up to 4 h. The effect of a P-glycoprotein transporter inhibitor, verapamil as a model drug, on the efficiency of the marker transport across the cell monolayer is thoroughly investigated. The analytical features of the proposed flow method for cell permeation studies in real time are critically compared against conventional batch-wise procedures and microfluidic devices.
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- $a Marques, Sara S $u Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, 500 05, Hradec Králové, Czech Republic. UCIBIO, REQUIMTE, Department of Chemistry, Faculty of Pharmacy, University of Porto, 4051-401, Porto, Portugal.
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