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Characterization of microbial siderophores by mass spectrometry

T. Pluháček, K. Lemr, D. Ghosh, D. Milde, J. Novák, V. Havlíček,

. 2015 ; 35 (1) : 35-47. [pub] 20150515

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't, Review

Persistent link   https://www.medvik.cz/link/bmc17001139

Siderophores play important roles in microbial iron piracy, and are applied as infectious disease biomarkers and novel pharmaceutical drugs. Inductively coupled plasma and molecular mass spectrometry (ICP-MS) combined with high resolution separations allow characterization of siderophores in complex samples taking advantages of mass defect data filtering, tandem mass spectrometry, and iron-containing compound quantitation. The enrichment approaches used in siderophore analysis and current ICP-MS technologies are reviewed. The recent tools for fast dereplication of secondary metabolites and their databases are reported. This review on siderophores is concluded with their recent medical, biochemical, geochemical, and agricultural applications in mass spectrometry context.

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$a Ghosh, Dipankar $u Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, 110067, India.
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$a Milde, David $u Department of Analytical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacky University, 17. listopadu 12, 771 46, Olomouc, Czech Republic.
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$a Novák, Jiří $u Institute of Microbiology, AS CR v.v.i., Videnska 1083, CZ 142 20, Prague 4, Czech Republic.
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$a Havlíček, Vladimír $u Department of Analytical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacky University, 17. listopadu 12, 771 46, Olomouc, Czech Republic. Institute of Microbiology, AS CR v.v.i., Videnska 1083, CZ 142 20, Prague 4, Czech Republic.
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