The principles of large screening strategies, which are developed by industrial companies, have been recently adopted by researchers in the fields of molecular biology and oncology as invaluable tools for translational medicine. The declining costs of laboratory robotic machines have allowed high-throughput screening to become more available to academic centres with limited resources. Here, we describe how a robotic conventional liquid handling system could be used on a daily basis in laboratories to obtain consistent and reproducible results. Our approach allowed us to quickly screen a panel of more than 20 tumorigenic and non-tumorigenic cell lines for their responses to hydroxyurea, which is a DNA-damaging anticancer therapeutic drug. The format of 384-well microplates was used for manual cell seeding, and the effect of hydroxyurea was screened at multiple concentrations. The fluorescence-based CyQuant assay was employed as the readout method to analyse the cellular DNA content. The effectiveness of our approach was demonstrated in the experimental results.

Centre of Biomolecular and Cellular Engineering International Clinical Research Centre St Anne's University Hospital Brno Brno Czech Republic

Institute of Biophysics Academy of Sciences of the Czech Republic v v i Brno Czech Republic

Institute of Biostatistics and Analyses Masaryk University Brno Czech Republic

National Centre for Biomolecular Research Faculty of Science and Department of Biology Faculty of Medicine Masaryk University Brno Czech Republic