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A capillary electrophoresis-mass spectrometry based method for the screening of β-secretase inhibitors as potential Alzheimer's disease therapeutics
J. Schejbal, L. Slezáčková, R. Řemínek, Z. Glatz,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, validační studie
Odkazy
PubMed
28153449
DOI
10.1016/j.chroma.2017.01.057
Knihovny.cz E-zdroje
- MeSH
- Alzheimerova nemoc farmakoterapie MeSH
- aspartátové endopeptidasy antagonisté a inhibitory MeSH
- elektroforéza kapilární metody MeSH
- HEK293 buňky MeSH
- hmotnostní spektrometrie metody MeSH
- inhibitory proteas analýza terapeutické užití MeSH
- kinetika MeSH
- lidé MeSH
- preklinické hodnocení léčiv metody MeSH
- sekretasy antagonisté a inhibitory MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- validační studie MeSH
In this work a novel capillary electrophoresis-mass spectrometry (CE-MS) based method was developed and validated for the assay of β-secretase (BACE1) activity as a potential target for Alzheimer's disease (AD) treatment. In contrast with the typically used Förster resonance energy transfer (FRET) assays, an unlabelled decapeptide derived from the amyloid precursor protein BACE1 site with the "Swedish mutation" was used as the substrate. The CE usage enabled the enzymatic reaction to be carried out in as small a volume as 100μL in 60min with sufficient yields of proteolytic product, which was subsequently separated in a bare fused silica capillary using 12.5% acetic acid as a background electrolyte and detected by MS. The limits of detection and quantitation were estimated using the signal to noise ratio to be 5nM (S/N=3) and 15nM (S/N=10), respectively, both being well below the working range for kinetic and inhibition studies. Its applicability for the kinetic study of BACE1 was demonstrated using optimized enzyme assay conditions and the estimated kinetic parameter values were confirmed by classic CE-UV analyses. The method was finally used for the main purpose for which it was developed - to screen BACE1 inhibitors as potential AD therapeutics. The resulting kinetic and inhibition parameters values were compared to those published in the literature, which were almost exclusively obtained by FRET based assays. These comparisons brought up several issues that are further discussed below and favour the application of an unlabelled substrate. The proposed CE-MS based method offers a high-throughput capability for new drug development.
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