Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

A proteomic approach to the development of DIVA ELISA distinguishing pigs infected with Salmonella Typhimurium and pigs vaccinated with a Salmonella Typhimurium-based inactivated vaccine

J. Gebauer, H. Kudlackova, M. Kosina, K. Kovarcik, R. Tesarik, A. Osvaldova, M. Faldyna, J. Matiasovic,

. 2016 ; 12 (1) : 252. [pub] 20161111

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17013410

BACKGROUND: Salmonella enterica serovar Typhimurium is one of the most common enteropathogenic bacteria found in pigs in Europe. In our previous work, we demonstrated the protective effects in suckling piglets when their dams had been vaccinated with an S. Typhimurium-based inactivated vaccine. This study is focused on a procedure leading to serological discrimination between vaccinated and infected pigs. As we supposed, distinct environment during natural infection and in bacterial cultures used for vaccine preparation led to a slightly different spectrum of expressed S. Typhimurium proteins. The examination of porcine antibodies produced after the experimental infection with S. Typhimurium or after vaccination with S. Typhimurium-based inactivated vaccine by affinity chromatography and mass spectrometry revealed differences in antibody response applicable for serological differentiation of infected from vaccinated animals. RESULTS: Antibodies against Salmonella SipB, SipD and SseB proteins were detected at much higher levels in post-infection sera in comparison with control and post-vaccination sera. On the other hand, proteins BamB, OppA and a fragment of FliC interacted with antibodies from post-vaccination sera with a much higher intensity than from control and post-infection sera. In addition, we constructed ELISA assays using post-infection antigen - SipB protein and post-vaccination antigen - FliC-fragment and evaluated them on a panel of individual porcine sera. CONCLUSIONS: The analysis of antibody response of infected and vaccinated pigs by proteomic tools enabled to identify S. Typhimurium antigens useful for distinguishing infected from vaccinated animals. This approach can be utilized in other challenges where DIVA vaccine and a subsequent serological assay are required, especially when genetic modification of a vaccine strain is not desirable.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17013410
003      
CZ-PrNML
005      
20170427114738.0
007      
ta
008      
170413s2016 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1186/s12917-016-0879-1 $2 doi
035    __
$a (PubMed)27835998
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Gebauer, Jan $u Department of Immunology, Veterinary Research Institute, Hudcova296/70, 62100, Brno, Czech Republic. gebauer@vri.cz. Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska267/2, 611 37, Brno, Czech Republic. gebauer@vri.cz.
245    12
$a A proteomic approach to the development of DIVA ELISA distinguishing pigs infected with Salmonella Typhimurium and pigs vaccinated with a Salmonella Typhimurium-based inactivated vaccine / $c J. Gebauer, H. Kudlackova, M. Kosina, K. Kovarcik, R. Tesarik, A. Osvaldova, M. Faldyna, J. Matiasovic,
520    9_
$a BACKGROUND: Salmonella enterica serovar Typhimurium is one of the most common enteropathogenic bacteria found in pigs in Europe. In our previous work, we demonstrated the protective effects in suckling piglets when their dams had been vaccinated with an S. Typhimurium-based inactivated vaccine. This study is focused on a procedure leading to serological discrimination between vaccinated and infected pigs. As we supposed, distinct environment during natural infection and in bacterial cultures used for vaccine preparation led to a slightly different spectrum of expressed S. Typhimurium proteins. The examination of porcine antibodies produced after the experimental infection with S. Typhimurium or after vaccination with S. Typhimurium-based inactivated vaccine by affinity chromatography and mass spectrometry revealed differences in antibody response applicable for serological differentiation of infected from vaccinated animals. RESULTS: Antibodies against Salmonella SipB, SipD and SseB proteins were detected at much higher levels in post-infection sera in comparison with control and post-vaccination sera. On the other hand, proteins BamB, OppA and a fragment of FliC interacted with antibodies from post-vaccination sera with a much higher intensity than from control and post-infection sera. In addition, we constructed ELISA assays using post-infection antigen - SipB protein and post-vaccination antigen - FliC-fragment and evaluated them on a panel of individual porcine sera. CONCLUSIONS: The analysis of antibody response of infected and vaccinated pigs by proteomic tools enabled to identify S. Typhimurium antigens useful for distinguishing infected from vaccinated animals. This approach can be utilized in other challenges where DIVA vaccine and a subsequent serological assay are required, especially when genetic modification of a vaccine strain is not desirable.
650    _2
$a zvířata $7 D000818
650    _2
$a protilátky bakteriální $x krev $7 D000907
650    _2
$a antigeny bakteriální $x metabolismus $7 D000942
650    _2
$a ELISA $x veterinární $7 D004797
650    12
$a proteomika $7 D040901
650    _2
$a salmonelová infekce u zvířat $x diagnóza $x imunologie $7 D012481
650    _2
$a salmonelové vakcíny $x imunologie $7 D022562
650    _2
$a Salmonella typhimurium $x genetika $7 D012486
650    _2
$a prasata $7 D013552
650    _2
$a nemoci prasat $x diagnóza $x imunologie $7 D013553
650    _2
$a inaktivované vakcíny $x imunologie $7 D015164
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kudlackova, Hana $u Department of Immunology, Veterinary Research Institute, Hudcova296/70, 62100, Brno, Czech Republic.
700    1_
$a Kosina, Marcel $u Bioveta a. s., Komenskeho212/12, 683 23, Ivanovice na Hane, Czech Republic.
700    1_
$a Kovarcik, Kamil $u Department of Virology, Veterinary Research Institute, Hudcova296/70, 62100, Brno, Czech Republic.
700    1_
$a Tesarik, Radek $u Department of Immunology, Veterinary Research Institute, Hudcova296/70, 62100, Brno, Czech Republic.
700    1_
$a Osvaldova, Alena $u Department of Immunology, Veterinary Research Institute, Hudcova296/70, 62100, Brno, Czech Republic. Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1/3, 612 42, Brno, Czech Republic.
700    1_
$a Faldyna, Martin $u Department of Immunology, Veterinary Research Institute, Hudcova296/70, 62100, Brno, Czech Republic.
700    1_
$a Matiasovic, Jan $u Department of Immunology, Veterinary Research Institute, Hudcova296/70, 62100, Brno, Czech Republic.
773    0_
$w MED00172950 $t BMC veterinary research $x 1746-6148 $g Roč. 12, č. 1 (2016), s. 252
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27835998 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20170413 $b ABA008
991    __
$a 20170427115058 $b ABA008
999    __
$a ok $b bmc $g 1199875 $s 974188
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 12 $c 1 $d 252 $e 20161111 $i 1746-6148 $m BMC veterinary research $n BMC vet. res. $x MED00172950
LZP    __
$a Pubmed-20170413

Find record

Citation metrics

Archiving options